eCite Digital Repository

Identification of loci and candidate genes controlling kernel weight in barley based on a population for which whole genome assemblies are available for both parents


Zhou, H and Luo, W and Gao, S and Ma, J and Zhou, M and Wei, Y and Zheng, Y and Liu, Y and Liu, C, Identification of loci and candidate genes controlling kernel weight in barley based on a population for which whole genome assemblies are available for both parents, Crop Journal pp. 1-8. ISSN 2095-5421 (2020) [Refereed Article]

PDF (In-press)

Copyright Statement

2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production andAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license (

DOI: doi:10.1016/j.cj.2020.07.010


Kernel weight (KW), together with kernel number per unit area, determines yield of cereal crops. Here, two barley recombinant inbred lines (RILs) populations with a shared parent were used to identify loci controlling KW. One is Baudin/AWCS276 (BA) for which a linkage map was available. Several large-effect QTL controlling KW were detected in this population. Another is Morex/AWCS276 (MA). A linkage map with 5273 makers formed 1454 clusters, was constructed by the genotyping by sequence (GBS) data of 201 RILs from this population. A single marker was selected to represent each of the clusters and the linkage map constructed with these markers covers a total length of 1022.4 cM with an average interval of approximately 0.7 cM between loci. Three of the large-effect loci controlling KW (located on 2HL, 6HL, and 7HL, respectively) identified from the BA population were also detected in the MA population under different environments. The locus on 6HL was detected in each of the experiments conducted for both populations thus was selected for developing near isogenic lines (NILs). Apart from KW, the two isolines for each pair of the putative NILs obtained showed no significant difference for any of the morphological characteristics assessed. The average difference in KW between the isolines for all the NILs obtained was about 15% based on assessments under both glasshouse and field conditions. Taken advantage that high quality genome assemblies for both Morex and AWCS276 are available, we identified candidate genes underlying two of the three loci based on an orthologous analysis. The NILs developed and the candidate genes identified in this study should facilitate the cloning and functional analysis of genes regulating KW in barley.

Item Details

Item Type:Refereed Article
Keywords:barley, kernel weight, near isogenic lines, AWCS276
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Agrochemicals and biocides (incl. application)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Gao, S (Mr Shang Gao)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:145374
Year Published:2020
Web of Science® Times Cited:3
Deposited By:TIA - Research Institute
Deposited On:2021-07-19
Last Modified:2021-09-30
Downloads:10 View Download Statistics

Repository Staff Only: item control page