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Quantitative proteomics provides an insight into germination-related proteins in the obligate biotrophic plant pathogen Spongospora subterranea

Citation

Balotf, S and Wilson, R and Tegg, RS and Nichols, DS and Wilson, CR, Quantitative proteomics provides an insight into germination-related proteins in the obligate biotrophic plant pathogen Spongospora subterranea, Environmental Microbiology Reports pp. 1-12. ISSN 1758-2229 (2021) [Refereed Article]

Copyright Statement

Copyright 2021 Society for Applied Microbiology and John Wiley & Sons Ltd

DOI: doi:10.1111/1758-2229.12955

Abstract

The soil-borne and obligate plant-associated nature of S. subterranea has hindered a detailed study of this pathogen and in particular, the regulatory pathways driving the germination of S. subterranea remain unknown. To better understand the mechanisms that control the transition from dormancy to germination, protein profiles between dormant and germination stimulant-treated resting spores were compared using label-free quantitative proteomics. Among the ~680 proteins identified 20 proteins were found to be differentially expressed during the germination of S. subterranea resting spores. Elongation factor Tu, histones (H2A and H15), proteasome and DJ-1_PfpI, involved in transcription and translation, were upregulated during the germination of resting spores. Downregulation of both actin and beta-tubulin proteins occurred in the germinating spores, indicating that the changes in the cell wall cytoskeleton may be necessary for the morphological changes during the germination of the resting spore in S. subterranea. Our findings provide new approaches for the study of these and similar recalcitrant micro-organisms provide the first insights into the basic protein components of S. subterranea spores. A better understanding of S. subterranea biology may lead to the development of novel approaches for the management of persistent soil inoculum.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Balotf, S (Mr Sadegh Balotf)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Nichols, DS (Dr David Nichols)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:145185
Year Published:2021
Funding Support:Australian Research Council (DP180103337)
Web of Science® Times Cited:1
Deposited By:TIA - Research Institute
Deposited On:2021-07-08
Last Modified:2021-09-01
Downloads:0

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