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Oropharyngeal carriage of hpl-containing Haemophilus haemolyticus predicts lower prevalence and density of NTHi colonisation in healthy adults

Citation

Atto, B and Kunde, D and Gell, DA and Tristram, S, Oropharyngeal carriage of hpl-containing Haemophilus haemolyticus predicts lower prevalence and density of NTHi colonisation in healthy adults, Pathogens, 10, (5) pp. 1-9. ISSN 2076-0817 (2021) [Refereed Article]


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Copyright 2021 by the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/pathogens10050577

Abstract

Nontypeable Haemophilus influenzae (NTHi) is a major respiratory pathogen that initiates infection by colonising the upper airways. Strategies that interfere with this interaction may therefore have a clinically significant impact on the ability of NTHi to cause disease. We have previously shown that strains of the commensal bacterium Haemophilus haemolyticus (Hh) that produce a novel haem-binding protein, haemophilin, can prevent NTHi growth and interactions with host cells in vitro. We hypothesized that natural pharyngeal carriage of Hh strains with the hpl open reading frame (Hh-hpl+) would be associated with a lower prevalence and/or density of NTHi colonisation in healthy individuals. Oropharyngeal swabs were collected from 257 healthy adults in Australia between 2018 and 2019. Real-time PCR was used to quantitatively compare the oropharyngeal carriage load of NTHi and Hh populations with the Hh-hpl+ or Hh-hpl- genotype. The likelihood of acquiring/maintaining NTHi colonisation status over a two- to six-month period was assessed in individuals that carried either Hh-hpl- (n = 25) or Hh-hpl+ (n = 25). Compared to carriage of Hh-hpl- strains, adult (18-65 years) and elderly (>65 years) participants that were colonised with Hh-hpl+ were 2.43 or 2.67 times less likely to carry NTHi in their oropharynx, respectively. Colonisation with high densities of Hh-hpl+ correlated with a low NTHi carriage load and a 2.63 times lower likelihood of acquiring/maintaining NTHi colonisation status between visits. Together with supporting in vitro studies, these results encourage further investigation into the potential use of Hh-hpl+ as a respiratory probiotic candidate for the prevention of NTHi infection.

Item Details

Item Type:Refereed Article
Keywords:Haemophilus haemolyticus, haem-binding protein, haemophilin, nontypeable Haemophilus influenzae, respiratory probiotic
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Atto, B (Miss Brianna Atto)
UTAS Author:Kunde, D (Dr Dale Kunde)
UTAS Author:Gell, DA (Dr David Gell)
UTAS Author:Tristram, S (Dr Stephen Tristram)
ID Code:145167
Year Published:2021
Deposited By:Health Sciences
Deposited On:2021-07-05
Last Modified:2021-09-08
Downloads:0

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