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Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments

Citation

Gray, J and Chandry, PS and Kaur, M and Kocharunchitt, C and Fanning, S and Bowman, JP and Fox, EM, Colonisation dynamics of Listeria monocytogenes strains isolated from food production environments, Scientific Reports, 11, (1) Article 12195. ISSN 2045-2322 (2021) [Refereed Article]


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Copyright 2021 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/s41598-021-91503-w

Abstract

Listeria monocytogenes is a ubiquitous bacterium capable of colonising and persisting within food production environments (FPEs) for many years, even decades. This ability to colonise, survive and persist within the FPEs can result in food product cross-contamination, including vulnerable products such as ready to eat food items. Various environmental and genetic elements are purported to be involved, with the ability to form biofilms being an important factor. In this study we examined various mechanisms which can influence colonisation in FPEs. The ability of isolates (n = 52) to attach and grow in biofilm was assessed, distinguishing slower biofilm formers from isolates forming biofilm more rapidly. These isolates were further assessed to determine if growth rate, exopolymeric substance production and/or the agr signalling propeptide influenced these dynamics and could promote persistence in conditions reflective of FPE. Despite no strong association with the above factors to a rapid colonisation phenotype, the global transcriptome suggested transport, energy production and metabolism genes were widely upregulated during the initial colonisation stages under nutrient limited conditions. However, the upregulation of the metabolism systems varied between isolates supporting the idea that L. monocytogenes ability to colonise the FPEs is strain-specific.

Item Details

Item Type:Refereed Article
Keywords:Listeria monocytogenes, food production environments
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Food sciences
Research Field:Food packaging, preservation and processing
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Food safety
UTAS Author:Kocharunchitt, C (Mr Chawalit Kocharunchitt)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
ID Code:145106
Year Published:2021
Deposited By:TIA - Research Institute
Deposited On:2021-07-01
Last Modified:2021-09-14
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