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Stages of embryonic development in the live-bearing fish, Gambusia holbrooki

Citation

Mousavi, SE and Patil, JG, Stages of embryonic development in the live-bearing fish, Gambusia holbrooki, Developmental Dynamics pp. 1-72. ISSN 1097-0177 (2021) [Refereed Article]


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Copyright Statement

Copyright 2021 American Association for Anatomy

DOI: doi:10.1002/dvdy.388

Abstract

Background

Divergent morphology and placentation of Poeciliids make them suitable model for investigating how evolutionary selection has altered and conserved the developmental mechanisms. However, there is limited description of their embryonic staging, despite representing a key evolutionary node that shares developmental strategy with placental vertebrates. Here we describe the embryonic developmental stages of Gambusia holbrooki from zygote to parturition using freshly harvested embryos.

Results

We defined 40 embryonic stages using a numbered (stages 0-39; zygote to parturition respectively) and named (grouped into seven periods that is, zygote, cleavage, blastula, gastrula, segmentation, pharyngula and parturition) staging system. Two sets of quantitative (ie, egg diameter, embryonic total length, otic vesicle closure index, heart rates, the number of caudal fin rays and elements) and qualitative (ie, three-dimensional analysis of images and key morphological criteria) data were acquired and used in combination to describe each stage. All 40 stages are separated by well-defined morphological traits, revealing developmental novelties that are influenced by narrow perivitelline space, placentation, internal gestation, and sex differentiation.

Conclusions

The principal diagnostic features described are quick, reliable, and easy to apply. This system will benefit researchers investigating molecular ontogeny, particularly sexual differentiation mechanisms in G. holbrooki.

Item Details

Item Type:Refereed Article
Keywords:embryogenesis, morphology, ontogeny, developmental events, viviparous, invasive fish
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Animal production
Research Field:Animal growth and development
Objective Division:Environmental Policy, Climate Change and Natural Hazards
Objective Group:Understanding climate change
Objective Field:Climate change models
UTAS Author:Mousavi, SE (Mr Seyed Ehsan Mousavi)
UTAS Author:Patil, JG (Dr Jawahar Patil)
ID Code:144892
Year Published:2021
Deposited By:Fisheries and Aquaculture
Deposited On:2021-06-18
Last Modified:2021-09-01
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