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Rapid changes in spectral composition after darkness influences nitric oxide, glucose and hydrogen peroxide production in the Antarctic diatom Fragilariopsis cylindrus

Citation

Kennedy, F and Martin, A and McMinn, A, Rapid changes in spectral composition after darkness influences nitric oxide, glucose and hydrogen peroxide production in the Antarctic diatom Fragilariopsis cylindrus, Polar Biology, 44 pp. 1289-1303. ISSN 0722-4060 (2021) [Refereed Article]

Copyright Statement

The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021

DOI: doi:10.1007/s00300-021-02867-8

Abstract

Ice-associated phototrophic taxa contribute significantly to Antarctic primary production and are crucial to ecosystem stability in the Southern Ocean. The quantity and quality of light required for photosynthesis is the single most influential driver of ice-associated algal communities. While the presence of ice and snow greatly reduces the irradiance reaching the ice-water interface, it is also the spectral quality that influences the phototrophy of ice-associated microalgae communities. Here we test the capability of three electrochemical microsensors to detect photosynthetically derived "stress" metabolites produced by the Antarctic diatom F. cylindrus. Following a period of dark incubation, this photo-physiological response differed with respect to the intensity and spectral quality of light during re-illumination. Exposure to blue light resulted in impairment in photosynthetic efficiency of PSII (Fv/Fm) and resulted in the production of nitric oxide (NO), hydrogen peroxide (H2O2) and glucose exudation. A similar trend in metabolite production was observed when subjected to white light, but not during red or green illumination. These results indicate that rapid exposure to light and variation in spectral composition can cause significant stress that can be quantified using H2O2, NO and glucose microsensors. This metabolic overflow was triggered by the disruption of normal photosynthetic electron flow and it is proposed that the detection of extracellular metabolites can be directly attributed to intracellular activity.

Item Details

Item Type:Refereed Article
Keywords:Antarctic, Fragilariopsis cylindrus, sea-ice algae, ice tank, microfluidics, diatom, micro- electrode
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Biochemistry and cell biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Kennedy, F (Dr Fraser Kennedy)
UTAS Author:Martin, A (Dr Andrew Martin)
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:144758
Year Published:2021
Deposited By:Ecology and Biodiversity
Deposited On:2021-06-08
Last Modified:2021-09-21
Downloads:0

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