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Integrated microfluidic devices fabricated in poly (methyl methacrylate) (PMMA) for on-site therapeutic drug monitoring of aminoglycosides in whole blood

Citation

Al-aqbi, ZT and Yap, YC and Li, F and Breadmore, MC, Integrated microfluidic devices fabricated in poly (methyl methacrylate) (PMMA) for on-site therapeutic drug monitoring of aminoglycosides in whole blood, Biosensors, 9, (1) Article 19. ISSN 2079-6374 (2019) [Refereed Article]


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Copyright Statement

Copyright 2019 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/bios9010019

Abstract

On-site therapeutic drug monitoring (TDM) is important for providing a quick and accurate dosing to patients in order to improve efficacy and minimize toxicity. Aminoglycosides such as amikacin, gentamicin, and tobramycin are important antibiotics that have been commonly used to treat infections of chronic bacterial infections in the urinary tract, lung, and heart. However, these aminoglycosides can lead to vestibular and auditory dysfunction. Therefore, TDM of aminoglycosides is important due to their ototoxicity and nephrotoxicity. Here, we have developed a hot embossed poly (methyl methacrylate) (PMMA) microfluidic device featuring an electrokinetic size and mobility trap (SMT) to purify, concentrate, and separate the aminoglycoside antibiotic drugs amikacin, gentamicin, and tobramycin. These drugs were separated successfully from whole blood within 3 min, with 30-fold lower detection limits compared to a standard pinched injection. The limit of detections (LOD) were 3.75 g/mL for gentamicin, 8.53 g/mL for amikacin, and 6.00 g/mL for tobramycin. These are sufficient to cover the therapeutic range for treating sepsis of 610 μg/mL gentamicin and tobramycin and 1220 μg/mL of amikacin. The device is simple and could be mass produced via embossing or injection molding approaches.

Item Details

Item Type:Refereed Article
Keywords:therapeutic drug monitoring (TDM), aminoglycosides, size and mobility traps (SMT), analytical chemistry
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Al-aqbi, ZT (Mr Zaidon Al-aqbi)
UTAS Author:Yap, YC (Dr Yiing Yap)
UTAS Author:Li, F (Mr Feng Li)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:143952
Year Published:2019
Funding Support:Australian Research Council (FT130100101)
Web of Science® Times Cited:9
Deposited By:Chemistry
Deposited On:2021-04-12
Last Modified:2022-08-29
Downloads:22 View Download Statistics

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