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Precise, accurate and user-independent blood collection system for dried blood spot sample preparation
journal contribution
posted on 2023-05-20, 22:46 authored by Neto, R, Gooley, A, Michael BreadmoreMichael Breadmore, Emily HilderEmily Hilder, Lapierre, FAn accurate and precise 3 μL blood collection and dispensing system is presented for the preparation of dried blood spot (DBS) samples. Using end-to-end glass capillaries in conjugation with pre-punched DBS pads, a blood micro collection system was developed to eliminate the haematocrit dispersion, widely associated with DBS technology, while providing better levels of accuracy and precision during sample preparation. This methodology is compared to traditional micro-volume blood collection systems, such as a pipette and a digitally controlled analytical syringe. Results showed that % of recovery for the capillary methodology was closer to 100% across the three haematocrit (HCT) levels tested and when prepared by two users (98 to 100% for capillaries, 78 to 104% for pipette and 93 to 97% for digital syringe) attesting a higher accuracy. Additionally, by taking advantage of the capillary action mechanism to collect and dispense autonomously the desired volume of blood onto the DBS pad, coefficients of variation between two individuals were significantly lower than with standard methodologies (capillaries - 0.05 to 0.77%, pipette - 12.71 to 18.53% and digital syringe - 0.72 to 1.77%). This alternate aspiration and dispensing methodology could be used by different users without compromising accuracy or precision when handling low volumes of blood during the pre-analytical steps.
Funding
Australian Research Council
Trajan Scientific Australia Pty Ltd
University of South Australia
History
Publication title
Analytical and Bioanalytical ChemistryVolume
410Issue
14Pagination
3315-3323ISSN
1618-2642Department/School
School of Natural SciencesPublisher
Springer-Verlag HeidelbergPlace of publication
Tiergartenstrasse 17, Heidelberg, Germany, D-69121Rights statement
Copyright 2018 Springer-Verlag GmbH Germany, part of Springer NatureRepository Status
- Restricted