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Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect


Chuang, Y-F and Phipps, AJ and Lin, F-L and Hecht, V and Hewitt, AW and Wang, P-Y and Liu, G-S, Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect, Cellular and Molecular Life Sciences, 78, (6) pp. 2683-2708. ISSN 1420-682X (2021) [Refereed Article]

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The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021

DOI: doi:10.1007/s00018-020-03725-2


The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system provides a groundbreaking genetic technology that allows scientists to modify genes by targeting specific genomic sites. Due to the relative simplicity and versatility of the CRISPR/Cas system, it has been extensively applied in human genetic research as well as in agricultural applications, such as improving crops. Since the gene editing activity of the CRISPR/Cas system largely depends on the efficiency of introducing the system into cells or tissues, an efficient and specific delivery system is critical for applying CRISPR/Cas technology. However, there are still some hurdles remaining for the translatability of CRISPR/Cas system. In this review, we summarized the approaches used for the delivery of the CRISPR/Cas system in mammals, plants, and aquacultures. We further discussed the aspects of delivery that can be improved to elevate the potential for CRISPR/Cas translatability.

Item Details

Item Type:Refereed Article
Keywords:CRISPR/Cas, gene delivery, gene editing, gene therapy
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genomics
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Chuang, Y-F (Dr Yu-Fan Chuang)
UTAS Author:Phipps, AJ (Dr Andrew Phipps)
UTAS Author:Lin, F-L (Dr Fan-Li Lin)
UTAS Author:Hecht, V (Dr Valerie Hecht)
UTAS Author:Hewitt, AW (Professor Alex Hewitt)
UTAS Author:Liu, G-S (Associate Professor Guei-Sheung Liu)
ID Code:143640
Year Published:2021
Funding Support:National Health and Medical Research Council (1185600)
Web of Science® Times Cited:13
Deposited By:Menzies Institute for Medical Research
Deposited On:2021-03-29
Last Modified:2021-05-03
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