eCite Digital Repository

Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004

Citation

Sanchez-Sarmiento, AM and Ruoppolo, V and Muelbert, MMC and Neto, JSF and Catao-Dias, JL, Serological screening for Brucella spp. and Leptospira spp. antibodies in southern elephant seals Mirounga leonina from Elephant Island, Antarctica, in 2003 and 2004, Diseases of Aquatic Organisms, 142 pp. 161-170. ISSN 0177-5103 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 Inter-Research

DOI: doi:10.3354/DAO03548

Abstract

Brucella spp. and Leptospira spp. antibodies were surveyed in 35 southern elephant seals (SESs) Mirounga leonina at Elephant Island (South Shetland Islands), western Antarctic peninsula, in the Austral summer of 2003 and 2004. The rose Bengal test and a commercial competitive ELISA (c-ELISA) were used to detect Brucella spp. exposure, and the microscopic agglutination test (MAT) with 22 live serovars was used to determine anti-Leptospira spp. antibodies. We found evidence of Brucella spp. exposure in 3 of 35 (8.6%) SESs tested via the c-ELISA displaying high percentage inhibition (PI), similar to other studies in pinnipeds in which Brucella spp. antibodies have been determined. Two of the 3 positives were pups (PI = 70.4 and 86.6%), while the third was an adult female (PI = 48.8%). The 3 c-ELISA positive SESs were additionally tested via the serum agglutination test but were found to be negative. All individuals were negative for antibodies against 22 Leptospira spp. serovars by MAT. These results contribute to the knowledge and monitoring of zoonotic pathogens with epizootic potential in Southern Ocean pinnipeds. Given the potential impact that pathogens may have on the abundance of wild (sometimes threatened and endangered) populations, constant monitoring and surveillance are required to prevent pathogen spread, particularly under forecast climate change scenarios.

Item Details

Item Type:Refereed Article
Keywords:competitive ELISA, brucellosis, leptospirosis, southern elephant seals, Phocidae, serology
Research Division:Biological Sciences
Research Group:Evolutionary biology
Research Field:Host-parasite interactions
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Muelbert, MMC (Dr Monica Muelbert)
ID Code:143581
Year Published:2020
Deposited By:Ecology and Biodiversity
Deposited On:2021-03-25
Last Modified:2021-06-25
Downloads:0

Repository Staff Only: item control page