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Dysfunctional gut microbiome networks in childhood IgE-mediated food allergy
Citation
Lee, KH and Guo, J and Song, Y and Ariff, A and O'Sullivan, M and Hales, B and Mullins, BJ and Zhang, G, Dysfunctional gut microbiome networks in childhood IgE-mediated food allergy, International Journal of Molecular Sciences, 22, (4) pp. 1-10. ISSN 1422-0067 (2021) [Refereed Article]
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Copyright Statement
Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/).
Abstract
The development of food allergy has been reported to be related with the changes in the gut
microbiome, however the specific microbe associated with the pathogenesis of food allergy remains
elusive. This study aimed to comprehensively characterize the gut microbiome and identify individual or group gut microbes relating to food-allergy using 16S rRNA gene sequencing with network
analysis. Faecal samples were collected from children with IgE-mediated food allergies (n = 33) and
without food allergy (n = 27). Gut microbiome was profiled by 16S rRNA gene sequencing. OTUs
obtained from 16S rRNA gene sequencing were then used to construct a co-abundance network using
Weighted Gene Co-expression Network Analysis (WGCNA) and mapped onto Kyoto Encyclopedia
of Genes and Genomes (KEGG) pathways. We identified a co-abundance network module to be
positively correlated with IgE-mediated food allergy and this module was characterized by a hub
taxon, namely Ruminococcaceae UCG-002 (phylum Firmicutes). Functional pathway analysis of all the
gut microbiome showed enrichment of methane metabolism and glycerolipid metabolism in the gut
microbiome of food-allergic children and enrichment of ubiquinone and other terpenoid-quinone
biosynthesis in the gut microbiome of non-food allergic children. We concluded that Ruminococcaceae
UCG-002 may play determinant roles in gut microbial community structure and function leading to
the development of IgE-mediated food allergy.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | 16S rRNA gene sequencing, food allergy, microbiome, WGCNA, Ruminococcaceae |
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Cell development, proliferation and death |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Diagnosis of human diseases and conditions |
| UTAS Author: | Song, Y (Dr Yong Song) |
| ID Code: | 143417 |
| Year Published: | 2021 |
| Web of Science® Times Cited: | 13 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2021-03-16 |
| Last Modified: | 2021-11-18 |
| Downloads: | 14 View Download Statistics |
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