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Antiviral activity of Cynodon dactylon on white spot syndrome virus (WSSV)-infected shrimp: an attempt to mitigate risk in shrimp farming

Citation

Howlader, P and Ghosh, AK and Islam, SS and Bir, J and Banu, GR, Antiviral activity of Cynodon dactylon on white spot syndrome virus (WSSV)-infected shrimp: an attempt to mitigate risk in shrimp farming, Aquaculture International, 28, (4) pp. 1725-1738. ISSN 0967-6120 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 Springer Nature Switzerland AG

DOI: doi:10.1007/s10499-020-00553-w

Abstract

White spot syndrome virus (WSSV) is the most contagious pathogen causing huge economic losses in the shrimp industry worldwide. Several medicinal plants are known to have antiviral activity through the inhibition of viral diseases in fish and shellfish. Hence, there is a need to investigate the ability of natural remedies like plant sources to mitigate this devastating disease in crustaceans. This study was carried out to test the antiviral activity of ethanolic extract of Cynodon dactylon on WSSV in tiger shrimp Penaeus monodon juveniles with an average weight of 13.541  2.927g. Different doses of the extract (75, 100, and 150mg/kg) were administrated in vivo through intramuscular injection. The antiviral activity was determined by observing survival rates, and WSSV infection was confirmed at the end of the experiment through polymerase chain reaction (PCR) identification. Before the in vivo experiment, presence of antiviral compounds ((+)-catechin, vanillic acid, syringic acid, (−)-epicatechin, p-coumaric acid, and quercetin bioactive compounds) in C. dactylon was confirmed through high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis. The results obtained in this study show that a dose of 100 to 150mg/kg body weight ethanolic extract of C. dactylon prevented the infection of WSSV marked by 100% survival and absence of WSSV-specific band using nested diagnostic PCR, thus demonstrating the suitability of ethanolic extract of C. dactylon as a possible prophylactic for the prevention of WSSV infections in shrimp culture.

Item Details

Item Type:Refereed Article
Keywords:Cynodondactylon, plant extract, white spot syndromevirus, crustaceans, survival , polymerase chain reaction
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Agricultural biotechnology
Research Field:Agricultural marine biotechnology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture crustaceans (excl. rock lobster and prawns)
UTAS Author:Islam, SS (Mr Shikder Islam)
ID Code:143197
Year Published:2020
Web of Science® Times Cited:2
Deposited By:Fisheries and Aquaculture
Deposited On:2021-03-04
Last Modified:2021-05-19
Downloads:0

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