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Low responders to endurance training exhibit impaired hypertrophy and divergent biological process responses in rat skeletal muscle

Citation

West, DWD and Doering, TM and Thompson, J-LM and Budiono, BP and Lessard, SJ and Koch, LG and Britton, SL and Steck, R and Byrne, NM and Brown, MA and Peake, JM and Ashton, KJ and Coffey, VG, Low responders to endurance training exhibit impaired hypertrophy and divergent biological process responses in rat skeletal muscle, Experimental Physiology, 106 pp. 714-725. ISSN 1469-445X (2021) [Refereed Article]

Copyright Statement

Copyright 2021 the authors

DOI: doi:10.1113/EP089301

Abstract

Divergent skeletal muscle phenotypes result from chronic resistance-type versus endurance-type contraction, reflecting the principle of training specificity. Our aim was to determine whether there is a common set of genetic factors that influence skeletal muscle adaptation to divergent contractile stimuli. Female rats were obtained from a genetically heterogeneous rat population and were selectively bred from high responders to endurance training (HRT) or low responders to endurance training (LRT; n = 6/group; generation 19). Both groups underwent 14 days of synergist ablation to induce functional overload of the plantaris muscle before comparison to non-overloaded controls of the same phenotype. RNA sequencing was performed to identify Gene Ontology biological processes with differential (LRT vs. HRT) gene set enrichment. We found that running distance, determined in advance of synergist ablation, increased in response to aerobic training in HRT but not LRT (65 26 vs.−6 18%, mean SD, P < 0.0001). The hypertrophy response to functional overload was attenuated in LRT versus HRT (20.1 5.6 vs. 41.6 16.1%, P = 0.015). Between-group differences were observed in the magnitude of response of 96 upregulated and 101 downregulated pathways. A further 27 pathways showed contrasting upregulation or downregulation in LRT versus HRT in response to functional overload. In conclusion, low responders to aerobic endurance training were also low responders for compensatory hypertrophy, and attenuated hypertrophy was associated with differential gene set regulation. Our findings suggest that genetic factors that underpin aerobic training maladaptation might also dysregulate the transcriptional regulation of biological processes that contribute to adaptation to mechanical overload.

Item Details

Item Type:Refereed Article
Keywords:heritable factors, molecular networks, skeletal muscle plasticity, specificity of adaptation
Research Division:Health Sciences
Research Group:Sports science and exercise
Research Field:Exercise physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Byrne, NM (Professor Nuala Byrne)
ID Code:143140
Year Published:2021
Deposited By:Health Sciences
Deposited On:2021-03-01
Last Modified:2021-06-23
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