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Hemadsorption immunosorbent technique for the detection of dengue immunoglobulin M antibody

Citation

Gunasegaran, K and Lim, TW and Ahmed, A and Aaskov, JG and Lam, SK and Pang, T, Hemadsorption immunosorbent technique for the detection of dengue immunoglobulin M antibody, Journal of Clinical Microbiology, 23, (1) pp. 170-4. ISSN 0095-1137 (1986) [Refereed Article]


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DOI: doi:10.1128/JCM.23.1.170-174.1986

Abstract

We developed a highly specific, sensitive, and economical hemadsorption immunosorbent technique for the detection of dengue-specific immunoglobulin M (IgM) antibody. The technique is based on the reaction of human sera with anti-human IgM immobilized onto a solid phase followed by the detection of dengue-specific IgM by the addition of a known quantity of dengue virus hemagglutinin and goose erythrocytes. Denguespecific IgM-positive sera showed hemadsorption. IgM antibody specific for dengue virus was detected in 22 of 39 (56%) convalescent-phase sera from primary dengue infections and 8 of 10 (80%) convalescent-phase sera from secondary dengue infections. Additionally, 32 of 76 single sera from patients were positive for dengue IgM; these sera were previously uninterpretable by the hemagglutination inhibition test, as only a single serum specimen was available. No false-positive results were obtained with sera that were negative by the hemagglutination inhibition test for dengue virus. Crude dengue virus hemagglutinin preparations could be used without purification. Dengue-specific IgG did not interfere with the results, nor was there any cross-reactivity between dengue hemagglutinins and IgM specific for other viruses. Some cross-reactivity of the dengue-specific IgM was observed with Japanese encephalitis virus hemagglutinins, but this did not present any problems in the interpretation of results. This test is specific, inexpensive, highly reproducible, and simple to perform.

Item Details

Item Type:Refereed Article
Keywords:Dengue virus; antibodies; hemadsorption; IgM
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Virology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
ID Code:142868
Year Published:1986
Web of Science® Times Cited:14
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-02-24
Downloads:0

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