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Elevated natural killer cell responses in mice infected with recombinant vaccinia virus encoding murine IL-2
journal contribution
posted on 2023-05-20, 21:06 authored by Gunasegaran KarupiahGunasegaran Karupiah, Coupar, BE, Andrew, ME, Boyle, DB, Phillips, SM, Mullbacher, A, Blanden, RV, Ramshaw, IAThe role of cytotoxic T cells and NK cells in the recovery of immunodeficient, athymic, nude mice infected with a recombinant vaccinia virus (VV) encoding murine IL-2 was investigated. Kinetic studies with the IL-2-encoding recombinant (VV-HA-IL2) and control (VV-HA-TK) viruses excluded a role for cytotoxic T cells but suggested the possible involvement of NK cells. In athymic nude mice given VV-HA-IL2, NK activity was at least threefold higher than mice infected with VV-HA-TK and this activity persisted for at least 6 days after infection. The effectors mediating the NK-like activity were asialo-GM1+ (as-GM1+), Thy1.2+/-, CD4- and CD8-, the phenotype of conventional NK cells. Elevated NK activity coincided with the rapid clearance of VV-HA-IL2 from ovaries of infected normal CBA/H mice but not from ovaries of CBA beige mice which had no detectable NK activity in spleens or ovaries. The expression of IL-2 in recombinant VV infection probably induces a cascade of immunologic effects of which elevated NK activity is one. We speculate that the chemoattractant and NK activity augmenting effects of IL-2 may contribute to recovery from VV-infection.
History
Publication title
Journal of ImmunologyVolume
144Pagination
290-8ISSN
0022-1767Department/School
Tasmanian School of MedicinePublisher
Amer Assoc ImmunologistsPlace of publication
9650 Rockville Pike, Bethesda, USA, Md, 20814Rights statement
Copyright 1990 The American Association of ImmunologistsRepository Status
- Restricted