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Inhibition of viral replication by interferon-gamma-induced nitric oxide synthase

journal contribution
posted on 2023-05-20, 21:05 authored by Gunasegaran KarupiahGunasegaran Karupiah, Xie, QW, Buller, RM, Nathan, C, Duarte, C, MacMicking, JD
Interferons (IFNs) induce antiviral activity in many cell types. The ability of IFN-gamma to inhibit replication of ectromelia, vaccinia, and herpes simplex-1 viruses in mouse macrophages correlated with the cells' production of nitric oxide (NO). Viral replication was restored in IFN-gamma-treated macrophages exposed to inhibitors of NO synthase. Conversely, epithelial cells with no detectable NO synthesis restricted viral replication when transfected with a complementary DNA encoding inducible NO synthase or treated with organic compounds that generate NO. In mice, an inhibitor of NO synthase converted resolving ectromelia virus infection into fulminant mousepox. Thus, induction of NO synthase can be necessary and sufficient for a substantial antiviral effect of IFN-gamma.

History

Publication title

Science

Volume

261

Issue

5127

Pagination

1445-8

ISSN

0036-8075

Department/School

Tasmanian School of Medicine

Publisher

Amer Assoc Advancement Science

Place of publication

1200 New York Ave, Nw, Washington, USA, Dc, 20005

Rights statement

Copyright © 1993, American Association for the Advancement of Science

Repository Status

  • Restricted

Socio-economic Objectives

Prevention of human diseases and conditions; Treatment of human diseases and conditions

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