eCite Digital Repository

Gamma interferon-induced, nitric oxide-mediated inhibition of vaccinia virus replication


Harris, N and Buller, RM and Karupiah, G, Gamma interferon-induced, nitric oxide-mediated inhibition of vaccinia virus replication, Journal of Virology, 69, (2) pp. 910-915. ISSN 0022-538X (1995) [Refereed Article]

Copyright Statement

Copyright 1995, American Society for Microbiology

DOI: doi:10.1128/JVI.69.2.910-915.1995


Gamma interferon (IFN-g)-induced nitric oxide synthase (iNOS) and nitric oxide (NO) production in the murine macrophage-like RAW 264.7 cells were previously shown to inhibit the replication of the poxviruses vaccinia virus (VV) and ectromelia virus and herpes simplex virus type 1. In the current study, we performed biochemical analyses to determine the stage in the viral life cycle blocked by IFN-g-induced NO. Antibodies specific for temporally expressed viral proteins, a VV-specific DNA probe, and transmission electron microscopy were used to show that the cytokine-induced NO inhibited late protein synthesis, DNA replication, and virus particle formation but not expression of the early proteins analyzed. Essentially similar results were obtained with hydroxyurea and cytosine arabinoside, inhibitors of DNA replication. Enzymatically active iNOS was detected in the lysates of IFN-g-treated but not in untreated RAW 264.7 cells. The IFN-g-treated RAW 264.7 cells which express iNOS not only were resistant to productive infection but also efficiently blocked the replication of VV in infected bystander cells of epithelial origin. This inhibition was arginine dependent, correlated with nitrite production in cultures, and was reversible by the NOS inhibitor Nv-monomethyl-Larginine.

Item Details

Item Type:Refereed Article
Keywords:IFN-g; Nitric oxide; inhibition of viral replication
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142852
Year Published:1995
Web of Science® Times Cited:149
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-05-25

Repository Staff Only: item control page