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Rapid Interferon g–dependent Clearance of Influenza A Virus and Protection from Consolidating Pneumonitis in Nitric Oxide Synthase 2–deficient Mice


Karupiah, G and Chen, J-H and Mahalingam, S and Nathan, CF and MacMicking, JD, Rapid Interferon g-dependent Clearance of Influenza A Virus and Protection from Consolidating Pneumonitis in Nitric Oxide Synthase 2-deficient Mice, The Journal of Experimental Medicine, 188, (8) pp. 1541-1546. ISSN 0022-1007 (1998) [Refereed Article]

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Author copyright 1998 Copyright The Rockefeller University Press

DOI: doi:10.1084/jem.188.8.1541


Viral infection often activates the interferon (IFN)-g–inducible gene, nitric oxide synthase 2 (NOS2). Expression of NOS2 can limit viral growth but may also suppress the immune system and damage tissue. This study assessed each of these effects in genetically deficient NOS22/2 mice after infection with influenza A, a virus against which IFN-g has no known activity. At inocula sufficient to cause consolidating pneumonitis and death in wild-type control mice, NOS22/2 hosts survived with little histopathologic evidence of pneumonitis. Moreover, they cleared influenza A virus from their lungs by an IFN-g–dependent mechanism that was not evident in wild-type mice. Even when the IFN-g–mediated antiviral activity was blocked in NOS22/2 mice with anti–IFN-g mAb, such mice failed to succumb to disease. Further evidence that this protection was independent of viral load was provided by treating NOS21/1 mice with the NOS inhibitor, Nv-methyl-l-arginine (l-NMA). l-NMA prevented mortality without affecting viral growth. Thus, host NOS2 seems to contribute more significantly to the development of influenza pneumonitis in mice than the cytopathic effects of viral replication. Although NOS2 mediates some antiviral effects of IFN-g, during influenza infection it can suppress another IFN-g–dependent antiviral mechanism. This mechanism was observed only in the complete absence of NOS2 activity and appeared sufficient to control influenza A virus growth in the absence of changes in cytotoxic T lymphocyte activity.

Item Details

Item Type:Refereed Article
Keywords:Nitric oxide; antiviral immunity; influenza A virus
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Innate immunity
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142845
Year Published:1998
Web of Science® Times Cited:154
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-05-27
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