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Enhanced antiviral antibody secretion and attenuated immunopathology during influenza virus infection in nitric oxide synthase-2-deficient mice

Citation

Jayasekera, JP and Vinuesa, GG and Karupiah, G and King, NJC, Enhanced antiviral antibody secretion and attenuated immunopathology during influenza virus infection in nitric oxide synthase-2-deficient mice, Journal of General Virology, 87, (Pt11) pp. 3361-3371. ISSN 0022-1317 (2006) [Refereed Article]


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DOI: doi:10.1099/vir.0.82131-0

Abstract

NOS2 gene-deficient (NOS2(-/-)) mice are less susceptible than wild-type (NOS2(+/+)) mice to infection with Influenza A virus. Virus titres in the lungs of influenza-infected NOS2(-/-) mice are significantly lower than those in NOS2(+/+) mice, with enhanced viral clearance in NOS2(-/-) mice dependent on gamma interferon (IFN-gamma). The current study was undertaken to ascertain the role of specific components of the immune response in promoting virus clearance in influenza-infected NOS2(-/-) mice. Levels of T cell- and natural killer cell-mediated cytotoxicity in the lungs of virus-infected mice were not significantly different between NOS2(+/+) and NOS2(-/-) mice. However, virus-infected NOS2(-/-) mice produced higher levels of virus-specific IgG2a antibody. Furthermore, more viable B cells and plasmablasts, along with greater levels of IFN-gamma, were found in NOS2(-/-) splenocyte cultures stimulated with B-cell mitogens. In addition to the early reduction in virus titres, clinical symptoms and proinflammatory cytokine production were attenuated in NOS2(-/-) mice. Thus, NOS2(-/-) B cells are capable of responding rapidly to influenza virus infection by proliferating and preferentially producing antibody of the IgG2a subtype. The relationship between viral load and the development of immunopathology is discussed.

Item Details

Item Type:Refereed Article
Keywords:Influenza A virus, nitric oxide, antibody responses, Lung pathology
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical virology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142823
Year Published:2006
Web of Science® Times Cited:28
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-02-23
Downloads:0

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