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Poxvirus CD8+ T-Cell determinants and cross-reactivity in BALB/c mice
journal contribution
posted on 2023-05-20, 21:00 authored by Tscharke, DC, Woo, W-P, Sakala, IG, Sidney, J, Sette, A, Moss, DJ, Bennink, JR, Gunasegaran KarupiahGunasegaran Karupiah, Yewdell, JWMouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and smallpox. However, some essential tools for their study that are taken for granted with other mouse models are not available for these viruses. Here we map and characterize the initial CD8+ T-cell determinants for poxviruses in H-2d-haplotype mice. CD8+ T cells recognizing these three determinants make up around 40% of the total responses to vaccinia virus during and after resolution of infection. We then use these determinants to test if predicted conservation across orthopoxvirus species matches experimental observation and find an unexpectedly cross-reactive variant peptide encoded by ectromelia (mousepox) virus.
History
Publication title
Journal of VirologyVolume
80Issue
13Pagination
6318-6323ISSN
0022-538XDepartment/School
Tasmanian School of MedicinePublisher
Amer Soc MicrobiologyPlace of publication
1752 N St Nw, Washington, USA, Dc, 20036-2904Rights statement
Copyright © 2006, American Society for MicrobiologyRepository Status
- Restricted