eCite Digital Repository
Whole-genome analyses reveal gene content differences between nontypeable Haemophilus influenzae isolates from chronic obstructive pulmonary disease compared to other clinical phenotypes
Citation
KC, R and Leong, KWC and Harkness, NM and Lachowicz, J and Gautam, SJ and Cooley, LA and McEwan, B and Petrovski, S and Karupiah, G and O'Toole, RF, Whole-genome analyses reveal gene content differences between nontypeable Haemophilus influenzae isolates from chronic obstructive pulmonary disease compared to other clinical phenotypes, Microbial Genomics, 6, (8) pp. 1-12. ISSN 2057-5858 (2020) [Refereed Article]
 | PDF Pending copyright assessment - Request a copy 3Mb |
DOI: doi:10.1099/mgen.0.000405
Abstract
Nontypeable Haemophilus influenzae (NTHi) colonizes human upper respiratory airways and plays a key role in the course and pathogenesis of acute exacerbations of chronic obstructive pulmonary disease (COPD). Currently, it is not possible to distinguish COPD isolates of NTHi from other clinical isolates of NTHi using conventional genotyping methods. Here, we analysed the core and accessory genome of 568 NTHi isolates, including 40 newly sequenced isolates, to look for genetic distinctions between NTHi isolates from COPD with respect to other illnesses, including otitis media, meningitis and pneumonia. Phylogenies based on polymorphic sites in the core-genome did not show discrimination between NTHi strains collected from different clinical phenotypes. However, pan-genome-wide association studies identified 79 unique NTHi accessory genes that were significantly associated with COPD. Furthermore, many of the COPD-related NTHi genes have known or predicted roles in virulence, transmembrane transport of metal ions and nutrients, cellular respiration and maintenance of redox homeostasis. This indicates that specific genes may be required by NTHi for its survival or virulence in the COPD lung. These results advance our understanding of the pathogenesis of NTHi infection in COPD lungs.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | COPD, nontypeable Haemophilus influenzae, pan-genome-wide association studies, whole-genome sequencing |
| Research Division: | Biological Sciences |
| Research Group: | Microbiology |
| Research Field: | Bacteriology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Prevention of human diseases and conditions |
| UTAS Author: | KC, R (Mr Rajendra KC) |
| UTAS Author: | Gautam, SJ (Mr Sanjay Gautam) |
| UTAS Author: | Karupiah, G (Associate Professor Guna Karupiah) |
| ID Code: | 142812 |
| Year Published: | 2020 |
| Deposited By: | Medicine |
| Deposited On: | 2021-02-12 |
| Last Modified: | 2021-02-22 |
| Downloads: | 0 |
Repository Staff Only: item control page