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Whole-genome analyses reveal gene content differences between nontypeable Haemophilus influenzae isolates from chronic obstructive pulmonary disease compared to other clinical phenotypes

Citation

KC, R and Leong, KWC and Harkness, NM and Lachowicz, J and Gautam, SJ and Cooley, LA and McEwan, B and Petrovski, S and Karupiah, G and O'Toole, RF, Whole-genome analyses reveal gene content differences between nontypeable Haemophilus influenzae isolates from chronic obstructive pulmonary disease compared to other clinical phenotypes, Microbial Genomics, 6, (8) pp. 1-12. ISSN 2057-5858 (2020) [Refereed Article]


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DOI: doi:10.1099/mgen.0.000405

Abstract

Nontypeable Haemophilus influenzae (NTHi) colonizes human upper respiratory airways and plays a key role in the course and pathogenesis of acute exacerbations of chronic obstructive pulmonary disease (COPD). Currently, it is not possible to distinguish COPD isolates of NTHi from other clinical isolates of NTHi using conventional genotyping methods. Here, we analysed the core and accessory genome of 568 NTHi isolates, including 40 newly sequenced isolates, to look for genetic distinctions between NTHi isolates from COPD with respect to other illnesses, including otitis media, meningitis and pneumonia. Phylogenies based on polymorphic sites in the core-genome did not show discrimination between NTHi strains collected from different clinical phenotypes. However, pan-genome-wide association studies identified 79 unique NTHi accessory genes that were significantly associated with COPD. Furthermore, many of the COPD-related NTHi genes have known or predicted roles in virulence, transmembrane transport of metal ions and nutrients, cellular respiration and maintenance of redox homeostasis. This indicates that specific genes may be required by NTHi for its survival or virulence in the COPD lung. These results advance our understanding of the pathogenesis of NTHi infection in COPD lungs.

Item Details

Item Type:Refereed Article
Keywords:COPD, nontypeable Haemophilus influenzae, pan-genome-wide association studies, whole-genome sequencing
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:KC, R (Mr Rajendra KC)
UTAS Author:Gautam, SJ (Mr Sanjay Gautam)
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:142812
Year Published:2020
Deposited By:Medicine
Deposited On:2021-02-12
Last Modified:2021-02-22
Downloads:0

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