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The interactive effect of dietary choline and water temperature on the liver lipid composition, histology, and plasma biochemistry of juvenile yellowtail kingfish (Seriola lalandi)

Citation

Liu, A and Pirozzi, I and Codabaccus, BM and Sammut, J and Booth, MA, The interactive effect of dietary choline and water temperature on the liver lipid composition, histology, and plasma biochemistry of juvenile yellowtail kingfish (Seriola lalandi), Aquaculture, 531 Article 735893. ISSN 0044-8486 (2021) [Refereed Article]

Copyright Statement

Copyright 2020 Elsevier B.V.

DOI: doi:10.1016/j.aquaculture.2020.735893

Abstract

The study examined the interactive effects of choline content (supplied as choline chloride salt (CC); no added CC, 3.0, or 6.0g CC kg−1 diet) and water temperature (16C vs 24C) on liver lipid composition, liver histology and plasma biochemistry. Liver and plasma samples were collected from juvenile yellowtail kingfish (Seriola lalandi; YTK; 156g body weight), fed fishmeal-based practical diets, at the conclusion of an eight-week feeding experiment. The results showed that higher liver lipid content at 24C was due to greater triacylglycerol (TAG) accumulation. Liver phospholipids content remained similar regardless of CC supplementation and temperature (P>0.05). As expected, liver phospholipids were mainly composed of phosphatidylcholine (PC); however, in most cases liver phospholipids were almost completely made up of PC. Phospholipid classes were also not significantly affected by CC supplementation and temperature. The results indicate that these lipid classes were tightly regulated in the liver of juvenile YTK. Fatty acids composition among TAG, FFA, and phospholipids fractions were different; however, within the phospholipids fraction, the long chain polyunsaturated fatty acids (LC-PUFA) proportion in the liver of YTK remained unchanged regardless of treatments. In general, histological examination did not show severe lesions in fish livers across all treatments. Fish also had significantly more hepatocytes with large nuclei at 16C than at 24C which is a response that requires further research. Plasma analytes were profoundly affected by temperature rather than by CC supplementation; however, there is no strong evidence indicating compromised health in fish reared at either temperature. This study demonstrates that fish fed fishmeal-based practical diets generally had normal looking livers; nevertheless, some liver and plasma health indicators can be affected in juvenile YTK reared at sub-optimal temperatures and fed diets without supplemented choline.

Item Details

Item Type:Refereed Article
Keywords:choline metabolism, lipid classes, fatty acids, histology, large nuclei, plasma biochemistry, nutrition
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Animal production
Research Field:Animal nutrition
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Codabaccus, BM (Dr Basseer Codabaccus)
ID Code:142717
Year Published:2021
Deposited By:Fisheries and Aquaculture
Deposited On:2021-02-09
Last Modified:2021-05-19
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