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High-throughput screening of biofilm formation of Listeria monocytogenes on stainless steel coupons using a 96-well plate format
chapter
posted on 2023-05-22, 18:45 authored by Gray, JA, Chandry, PS, John BowmanJohn Bowman, Fox, EMListeria monocytogenes is a foodborne pathogen capable of colonizing and persisting in the food production environment (FPE). While there are a variety of factors involved in L. monocytogenes’ ability to persist in FPE, the ability to form biofilms has the potential to increase their chance of survival and long-term colonization. Understanding the mechanisms involved in L. monocytogenes ability to form biofilms may potentially help food safety managers optimize control strategies targeting it in the FPE. In this chapter, a high-throughput method to determine L. monocytogenes ability to attach and form biofilms utilizing FPE-grade stainless steel is described. This method provides fast and efficient results, facilitating scaling up to large numbers of isolates to measure their ability to form biofilms, where lower-throughput approaches can then be utilized to further characterize isolates of interest.
Funding
CSIRO-Commonwealth Scientific & Industrial Research Organisation
History
Publication title
Listeria Monocytogenes: Methods and ProtocolsVolume
2220Editors
EM Fox, H Bierne & B StesslPagination
115-122ISBN
978-1-0716-0982-8Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Humana Press/Springer NaturePlace of publication
New York, USAExtent
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Copyright 2021 Springer Science+Business Media, LLC, part of Springer NatureRepository Status
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