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High-throughput screening of biofilm formation of Listeria monocytogenes on stainless steel coupons using a 96-well plate format

Citation

Gray, JA and Chandry, PS and Bowman, JP and Fox, EM, High-throughput screening of biofilm formation of Listeria monocytogenes on stainless steel coupons using a 96-well plate format, Listeria Monocytogenes: Methods and Protocols, Humana Press/Springer Nature, EM Fox, H Bierne & B Stessl (ed), New York, USA, pp. 115-122. ISBN 978-1-0716-0982-8 (2021) [Research Book Chapter]

Copyright Statement

Copyright 2021 Springer Science+Business Media, LLC, part of Springer Nature

DOI: doi:10.1007/978-1-0716-0982-8_9

Abstract

Listeria monocytogenes is a foodborne pathogen capable of colonizing and persisting in the food production environment (FPE). While there are a variety of factors involved in L. monocytogenes ability to persist in FPE, the ability to form biofilms has the potential to increase their chance of survival and long-term colonization. Understanding the mechanisms involved in L. monocytogenes ability to form biofilms may potentially help food safety managers optimize control strategies targeting it in the FPE. In this chapter, a high-throughput method to determine L. monocytogenes ability to attach and form biofilms utilizing FPE-grade stainless steel is described. This method provides fast and efficient results, facilitating scaling up to large numbers of isolates to measure their ability to form biofilms, where lower-throughput approaches can then be utilized to further characterize isolates of interest.

Item Details

Item Type:Research Book Chapter
Keywords:biofilm formation, high-throughput, stainless steel, 96-well microtiter plate, Listeria monocytogenes, methodology
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Food sciences
Research Field:Food safety, traceability, certification and authenticity
Objective Division:Health
Objective Group:Public health (excl. specific population health)
Objective Field:Food safety
UTAS Author:Bowman, JP (Associate Professor John Bowman)
ID Code:142665
Year Published:2021
Deposited By:TIA - Research Institute
Deposited On:2021-02-04
Last Modified:2021-09-06
Downloads:0

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