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Comparison of bacterial diversity and distribution on the gills of Atlantic salmon (Salmo salar L.): an evaluation of sampling techniques

Citation

Slinger, J and Adams, MB and Wynne, JW, Comparison of bacterial diversity and distribution on the gills of Atlantic salmon (Salmo salar L.): an evaluation of sampling techniques, Journal of Applied Microbiology pp. 1-13. ISSN 1364-5072 (2020) [Refereed Article]


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DOI: doi:10.1111/jam.14969

Abstract

Aims

Assess bacterial diversity and richness in mucus samples from the gills of Atlantic salmon in comparison to preserved or fixed gill filament tissues. Ascertain whether bacterial diversity and richness are homogeneous upon different arches of the gill basket.

Methods and Results

Bacterial communities contained within gill mucus were profiled using 16S rRNA gene sequencing. No significant difference in taxa richness, alpha (P > 005) or beta diversity indices (P > 005) were found between the bacterial communities of RNAlater preserved gill tissues and swab‐bound mucus. A trend of lower richness and diversity indices were observed in bacterial communities from posterior hemibranchs.

Conclusions

Non‐lethal swab sampling of gill mucus provides a robust representation of bacterial communities externally upon the gills. Bacterial communities from the fourth arch appeared to be the least representative overall.

Significance and Impact of the Study

The external mucosal barriers of teleost fish (e.g. gill surface) play a vital role as a primary defence line against infection. While research effort on the role of microbial communities on health and immunity of aquaculture species continues, the collection and sampling processes to obtain these data require evaluation so methodologies are consistently applied across future studies that aim to evaluate the composition of branchial microbiomes.

Item Details

Item Type:Refereed Article
Keywords:amoebic gill disease, gill bacteria, microbiome, salmon
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture crustaceans (excl. rock lobster and prawns)
UTAS Author:Slinger, J (Mr Joel Slinger)
UTAS Author:Adams, MB (Dr Mark Adams)
ID Code:142396
Year Published:2020
Deposited By:Fisheries and Aquaculture
Deposited On:2021-01-14
Last Modified:2021-01-19
Downloads:0

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