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A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests

Citation

Pandigamage, P and Wilson, R and Quirino, JP, A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests, Journal of Chromatography A, 1628 Article 461443. ISSN 0021-9673 (2020) [Refereed Article]

Copyright Statement

Crown Copyright 2020

DOI: doi:10.1016/j.chroma.2020.461443

Abstract

Sodium dodecyl sulfate (SDS) in proteomics samples needs to be removed and estimated prior to mass spectrometry (MS)-based analysis and to avoid MS ion-source contamination. Here, we describe an organic solvent free method to remove SDS using a simple apparatus that mainly consists of an agarose gel inside a 1 mL plastic micropipette tip and a voltage power supply with electrodes. A small volume of sample (e.g., 50 μL) is loaded on top of the gel and then voltage (cathode at the sample side) is applied with an acidic solution at the other end of the micropipette tip. Within 25 min, SDS was removed (e.g., ≥99% SDS in 3.5 mM SDS) and the peptides were retained in the sample solution. The strategy was compared to the commercially available and expensive Pierce spin column for the removal of SDS and recovery of peptides from a digested bovine serum albumin sample.

Item Details

Item Type:Refereed Article
Keywords:sodium dodecyl sulfate, proteomics, protein digestion, electrophoresis
Research Division:Chemical Sciences
Research Group:Medicinal and biomolecular chemistry
Research Field:Proteins and peptides
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Pandigamage, P (Mr Pravinda Pandigamage)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:142263
Year Published:2020
Deposited By:Central Science Laboratory
Deposited On:2021-01-04
Last Modified:2021-02-23
Downloads:0

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