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In utero exposure to diesel exhaust particles, but not silica, alters post-natal immune development and function

Citation

Thaver, S and Foa, L and Richards, SM and Lyons, AB and Zosky, GR, In utero exposure to diesel exhaust particles, but not silica, alters post-natal immune development and function, Chemosphere, 268 Article 1293142. ISSN 0045-6535 (2020) [Refereed Article]


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DOI: doi:10.1016/j.chemosphere.2020.129314

Abstract

Our understanding of the impact of in utero exposure to PM on post-natal immune function and the subsequent response to PM exposure is limited. Similarly, very few studies have considered the effect of exposure to PM from different sources. Thus, the aim of this study was to examine how in utero exposure to PM from different sources effects the post-natal response to pro-inflammatory and immune stimuli. C56BL/6J pregnant mice were exposed intranasally on gestational day (E)7.5, E12.5 and E17.5-50 μg of diesel exhaust particles (DEP), silica or saline. At 4-weeks post-natal age, sub-groups of male and female mice were exposed intranasally to 50 μg of DEP or saline. Lung inflammatory responses were assessed 6 h later by quantifying inflammatory cells and cytokine production (MCP-1, MIP-2, IL-6). In separate groups of mice, the spleen was harvested to quantify B and T cell populations. Splenocytes were isolated and exposed to lipopolysaccharide or poly I:C for assessment of cytokine production. Exposure to DEP in utero decreased %CD1dhighCD5+ B cells in female mice and IFN-γ production by splenocytes in both sexes. Male mice had elevations in macrophage and lymphocyte numbers in response to DEP whereas female mice only had elevated IL-6, MCP-1 and MIP-2 levels. In utero exposure to silica had no effect on these measures. These data suggest that in utero exposure to PM alters immune development and post-natal immune function. This response is dependent on the source of PM, which has implications for understanding the community health effects of exposure to air pollution.

Item Details

Item Type:Refereed Article
Keywords:particulate matter, silica, diesel, in utero, immune development, diesel exhaust particles
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Prevention of human diseases and conditions
UTAS Author:Thaver, S (Mr Santon Thaver)
UTAS Author:Foa, L (Professor Lisa Foa)
UTAS Author:Richards, SM (Dr Stephen Richards)
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:142155
Year Published:2020
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-12-16
Last Modified:2021-02-16
Downloads:0

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