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A new lymphoid-primed progenitor marked by Dach1 downregulation identified with single cell multi-omics
Citation
Amann-Zalcenstein, D and Tian, L and Schreuder, J and Tomei, S and Lin, DS and Fairfax, KA and Bolden, JE and McKenzie, MD and Jarratt, A and Hilton, A and Jackson, JT and Di Rago, L and McCormack, MP and de Graaf, CA and Stonehouse, O and Taoudi, S and Alexander, WS and Nutt, SL and Ritchie, ME and Ng, AP and Naik, SH, A new lymphoid-primed progenitor marked by Dach1 downregulation identified with single cell multi-omics, Nature Immunology, 21, (12) pp. 1574-1584. ISSN 1529-2908 (2020) [Refereed Article]
Copyright Statement
Copyright 2020 Nature Publishing Group
DOI: doi:10.1038/s41590-020-0799-x
Abstract
A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. Lin-c-Kit+Sca-1+Flt3+ cells, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate. Here, through single-cell RNA sequencing, we identify the expression of Dach1 and associated genes in this fraction as being coexpressed with myeloid/stem genes but inversely correlated with lymphoid genes. Through generation of Dach1-GFP reporter mice, we identify a transcriptionally and functionally unique Dach1-GFP- subpopulation within LMPPs with lymphoid potential with low to negligible classic myeloid potential. We term these 'lymphoid-primed progenitors' (LPPs). These findings define an early definitive branch point of lymphoid development in hematopoiesis and a means for prospective isolation of LPPs.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Immunology |
| Research Field: | Cellular immunology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Diagnosis of human diseases and conditions |
| UTAS Author: | Fairfax, KA (Dr Kirsten Fairfax) |
| ID Code: | 142014 |
| Year Published: | 2020 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2020-12-08 |
| Last Modified: | 2021-02-16 |
| Downloads: | 0 |
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