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Reproducibility of saliva progesterone measured by immunoassay compared to liquid chromatography mass spectrometry
Citation
Ney, LJ and Felmingham, KL and Nichols, D, Reproducibility of saliva progesterone measured by immunoassay compared to liquid chromatography mass spectrometry, Analytical Biochemistry, 610 Article 113984. ISSN 0003-2697 (2020) [Refereed Article]
Copyright Statement
Copyright 2020 Elsevier Inc.
DOI: doi:10.1016/j.ab.2020.113984
Abstract
Immunoassay overestimates progesterone in blood, but no studies have tested whether this occurs in saliva. We measured progesterone in saliva using immunoassay and mass spectrometry. We tested the immunoassay for cross reactivity with dehydroepiandrosterone sulfate (DHEA-S) and 17α-hydroxyprogesterone (17α-OHP). Progesterone was significantly higher in immunoassay compared to mass spectrometry. Immunoassay progesterone levels increased in when incremental levels of 17α-OHP standard was added. This effect was not observed with the addition of DHEA-S. Research using salivary progesterone immunoassay techniques should be wary, particularly with individuals taking steroid supplementation or with high levels of progesterone metabolites.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | progesterone, immunoassay, mass spectrometry, saliva, DHEA-S, 17a-OHP |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Clinical sciences |
| Research Field: | Clinical chemistry (incl. diagnostics) |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Diagnosis of human diseases and conditions |
| UTAS Author: | Ney, LJ (Mr Luke Ney) |
| UTAS Author: | Nichols, D (Dr David Nichols) |
| ID Code: | 141756 |
| Year Published: | 2020 |
| Deposited By: | Central Science Laboratory |
| Deposited On: | 2020-11-17 |
| Last Modified: | 2021-02-22 |
| Downloads: | 0 |
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