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Characterisation of the circulating and tissue-specific alterations to the lipidome in response to moderate and major cold stress in mice

Citation

Pernes, G and Morgan, PK and Huynh, K and Mellett, NA and Meikle, PJ and Murphy, AJ and Henstridge, DC and Lancaster, GI, Characterisation of the circulating and tissue-specific alterations to the lipidome in response to moderate and major cold stress in mice, American Journal of Physiology ISSN 0363-6119 (2020) [Refereed Article]


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Copyright Statement

Copyright 2020 American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

DOI: doi:10.1152/ajpregu.00112.2020

Abstract

This study analysed the effects of 24 hours of cold stress (22C or 5C vs. mice maintained at 30C) on the plasma, brown adipose tissue (BAT), subcutaneous (SubQ) and epididymal (Epi) white adipose tissue (WAT), liver, and skeletal muscle lipidome of mice. Using mass spectrometry-lipidomics 624 lipid species were detected, of which 239 were significantly altered in plasma, 134 in BAT, and 51 in the liver. In plasma, acylcarnitines and free fatty acids were markedly increased at 5C. Plasma triacylglycerols (TGs) were reduced at 22C and 5C. We also identified ether lipids as a novel, cold-induced lipid class. In BAT, TGs were the principal lipid class affected by cold stress, being significantly reduced at both 22C and 5C. Interestingly, while BAT TG species were uniformly affected at 5C, at 22C we observed species-dependent effects, with TGs containing longer and more unsaturated fatty acids particularly sensitive to the effects of cold. In the liver, TGs were the most markedly affected lipid class, increasing in abundance at 5C. TGs containing longer and more unsaturated fatty acids accumulated to a greater degree. Our work demonstrates: (1) Acute exposure to moderate (22C) cold stress alters the plasma and BAT lipidome; although this effect is markedly less pronounced than at 5C. (2) Cold stress at 5C dramatically alters the plasma lipidome, with ether lipids identified as a novel lipid class altered by cold exposure. (3) That cold-induced alterations in liver and BAT TG levels are not uniform, with changes being influenced by acyl chain composition.

Item Details

Item Type:Refereed Article
Keywords:lipids, obesity, diabetes, thermoneutrality
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Henstridge, DC (Mr Darren Henstridge)
ID Code:141746
Year Published:2020
Deposited By:Health Sciences
Deposited On:2020-11-16
Last Modified:2020-12-11
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