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Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB


Haustead, HJ and Stevenson, A and Saxena, V and Marriage, F and Firth, M and Silla, R and Martin, L and Adcroft, KF and Rea, S and Day, PJ and Melton, PE and Wood, FM and Fear, MW, Transcriptome analysis of human ageing in male skin shows mid-life period of variability and central role of NF-κB, Scientific Reports, 6 pp. 1-12. ISSN 2045-2322 (2016) [Refereed Article]

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DOI: doi:10.1038/srep26846


Age is well-known to be a significant factor in both disease pathology and response to treatment, yet the molecular changes that occur with age in humans remain ill-defined. Here, using transcriptome profiling of healthy human male skin, we demonstrate that there is a period of significantly elevated, transcriptome-wide expression changes occurring predominantly in middle age. Both pre and post this period, the transcriptome appears to undergo much smaller, linear changes with increasing age. Functional analysis of the transient changes in middle age suggest a period of heightened metabolic activity and cellular damage associated with NF-kappa-B and TNF signaling pathways. Through metaanalysis we also show the presence of global, tissue independent linear transcriptome changes with age which appear to be regulated by NF-kappa-B. These results suggest that aging in human skin is associated with a critical mid-life period with widespread transcriptome changes, both preceded and proceeded by a relatively steady rate of linear change in the transcriptome. The data provides insight into molecular changes associated with normal aging and will help to better understand the increasingly important pathological changes associated with aging.

Item Details

Item Type:Refereed Article
Keywords:gene expression, wound healing, skin, males, biological pathways
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene expression (incl. microarray and other genome-wide approaches)
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Melton, PE (Dr Phillip Melton)
ID Code:141481
Year Published:2016
Web of Science® Times Cited:25
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-10-22
Last Modified:2022-08-19
Downloads:11 View Download Statistics

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