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Spatio-temporal variability in the photosynthetic characteristics of Zostera tasmanica measured by PAM

Citation

Strain, E and Beardall, J and Thomson, R and Roberts, S and Light, B, Spatio-temporal variability in the photosynthetic characteristics of Zostera tasmanica measured by PAM, Aquatic Botany, 85, (1) pp. 21-28. ISSN 0304-3770 (2006) [Refereed Article]

Copyright Statement

Copyright 2006 Elsevier B.V.

DOI: doi:10.1016/j.aquabot.2006.01.009

Abstract

Rapid light curves (RLCs), based on pulse amplitude modulated (PAM) fluorometry, were used to investigate the spatio-temporal variability in photosynthesis versus irradiance parameters (α, Ik and Pmax) and the Fv/Fm ratio of the seagrass Zostera tasmanica (formerly Heterozostera tasmanica). Spatial variation was examined across scales ranging from within a leaf (cms) to across the bed (ms), using a nested analysis of covariance sampling design. Overall, significant variation was identified at all scales examined, excluding the largest scale (area). Patterns of variability differed among individual parameters; however a high percentage of the variation was consistently assigned to the covariates, age (within and between leaves) for all parameters, except Pmax.

Using these results, a sampling strategy focusing on the leaf level was used to examine temporal changes to the photosynthesis parameters and the Fv/Fm ratio and to minimize spatial considerations. Both Pmax and the Fv/Fm ratio showed a significant relationship with in situ irradiance. However, a large percentage of the variation, for all parameters, was left unexplained by in situ irradiance. When the effect of in situ irradiance was removed there was no autocorrelation between measurements, taken at 3 min intervals, of the photosynthesis parameters and the Fv/Fm ratio. These results suggest that small-scale spatial and not temporal variation must be taken into account when seeking to characterise Z. tasmanica photosynthetic performance using PAM fluorometry.

Item Details

Item Type:Refereed Article
Keywords:Zostera tasmanica, PAM fluorometry, RLCs, photosynthesis parameters (Ik, a, Pmax), Fv/Fm ratio, spatio-temporal variation
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Environmental Management
Objective Group:Marine systems and management
Objective Field:Assessment and management of benthic marine ecosystems
UTAS Author:Strain, E (Dr Beth Strain)
UTAS Author:Thomson, R (Dr Russell Thomson)
ID Code:141427
Year Published:2006
Web of Science® Times Cited:8
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2020-10-20
Last Modified:2020-11-17
Downloads:0

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