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Role of ligand purity in separations of alkaline earth metals as arsenazo I complexes by capillary zone electrophoresis


Macka, M and Paull, B and Bogan, DP and Haddad, PR, Role of ligand purity in separations of alkaline earth metals as arsenazo I complexes by capillary zone electrophoresis, Journal of Chromatography A, 793, (1) pp. 177-185. ISSN 0021-9673 (1998) [Refereed Article]

DOI: doi:10.1016/S0021-9673(97)00868-6


In the separation of metal ions by capillary electrophoresis in the form of kinetically labile complexes formed through the incorporation of an auxiliary complexing ligand into the background electrolyte (BGE), it has been shown that the purity of this auxiliary complexing ligand can play a crucial role in the selectivity and efficiency of the resultant separations. Using the separation of alkaline earth metals as complexes with the metallochromic ligand arsenazo I as a model system, the effects of the addition of low concentrations of simulated impurities, in the form of various metal ions and competing ligands, were studied. Addition of Fe(III) at low micromolar levels to a BGE containing 1 mM arsenazo I resulted in severe peak tailing. The addition of the competing ligands diethylenetriaminepentaacetic acid (DTPA) or arsenazo III, at a molar ratio as low as 1:1000 to arsenazo I, also caused substantial peak broadening and altered the separation selectivity. The practical implications of the above results for the separation of metals as labile complexes, using capillary zone electrophoresis (CZE) systems similar to the above, are discussed.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Instrumental methods (excl. immunological and bioassay methods)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Paull, B (Professor Brett Paull)
UTAS Author:Bogan, DP (Dr Declan Bogan)
UTAS Author:Haddad, PR (Professor Paul Haddad)
ID Code:14140
Year Published:1998
Web of Science® Times Cited:11
Deposited By:Chemistry
Deposited On:1998-08-01
Last Modified:2011-08-09

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