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Characterization and genome analysis of a novel marine Alteromonas phage P24


Zhang, X and Liu, Y and Wang, M and Wang, M and Jiang, T and Sun, J and Gao, C and Jiang, Y and Guo, C and Shao, H and Liang, Y and McMinn, A, Characterization and genome analysis of a novel marine Alteromonas phage P24, Current Microbiology, 77 pp. 2813-2820. ISSN 0343-8651 (2020) [Refereed Article]

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Copyright 2020 Springer Science+Business Media, LLC, part of Springer Nature

DOI: doi:10.1007/s00284-020-02077-1


Although Alteromonas is ubiquitous in the marine environment, very little is known about Alteromonas phages, with only ten, thus far, being isolated and reported on. In this study, a novel double-stranded DNA phage, Alteromonas phage P24, which infects Alteromonas macleodii, was isolated from the coastal waters off Qingdao. Alteromonas phage P24 has a siphoviral morphology, with an icosahedral head, 61 ± 1 nm in diameter, and a tail length of 105 ± 1 nm. Alteromonas phage P24 contains lipids. It has an optimal temperature and pH for growth of 20℃ and 5–7, respectively. A one-step growth curve shows a latent period of 55 min, a rise period of 65 min, and an average burst size of approximately 147 virions per cell. Alteromonas phage P24 has the genome of 46,945 bp with 43.80% GC content and 74 open reading frames (ORFs) without tRNA. The results of the phylogenetic tree, based on the mcp and terL genes, show that Alteromonas phage P24 is closely related to Aeromonas phage phiARM81ld. Meanwhile, phylogenetic analysis based on the whole genome of P24 indicates that it forms a unique viral sub-cluster within Siphoviridae. This study contributes to the understanding of the genomic characteristics and the virus-host interactions of Alteromonas phages.

Item Details

Item Type:Refereed Article
Keywords:virus, Alteromonas, genome
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Virology
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Coastal or estuarine biodiversity
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:141271
Year Published:2020
Web of Science® Times Cited:11
Deposited By:Ecology and Biodiversity
Deposited On:2020-10-09
Last Modified:2021-02-17

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