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Double-stranded RNA binding proteins in serum contribute to systemic RNAi across phyla - towards finding the missing link in Achelata

Citation

Banks, TM and Wang, T and Fitzgibbon, QP and Smith, GG and Ventura, T, Double-stranded RNA binding proteins in serum contribute to systemic RNAi across phyla - towards finding the missing link in Achelata, International Journal of Molecular Sciences, 21, (18) Article 6967. ISSN 1422-0067 (2020) [Refereed Article]


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Copyright Statement

2020 by the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms21186967

Abstract

RNA interference (RNAi) has become a widely utilized method for studying gene function, yet despite this many of the mechanisms surrounding RNAi remain elusive. The core RNAi machinery is relatively well understood, however many of the systemic mechanisms, particularly double-stranded RNA (dsRNA) transport, are not. Here, we demonstrate that dsRNA binding proteins in the serum contribute to systemic RNAi and may be the limiting factor in RNAi capacity for species such as spiny lobsters, where gene silencing is not functional. Incubating sera from a variety of species across phyla with dsRNA led to a gel mobility shift in species in which systemic RNAi has been observed, with this response being absent in species in which systemic RNAi has never been observed. Proteomic analysis suggested lipoproteins may be responsible for this phenomenon and may transport dsRNA to spread the RNAi signal systemically. Following this, we identified the same gel shift in the slipper lobster Thenus australiensis and subsequently silenced the insulin androgenic gland hormone, marking the first time RNAi has been performed in any lobster species. These results pave the way for inducing RNAi in spiny lobsters and for a better understanding of the mechanisms of systemic RNAi in Crustacea, as well as across phyla.

Item Details

Item Type:Refereed Article
Keywords:decapod crustaceans, dsRNA transport, gene silencing mechanism, serum dsRNA binding proteins, systemic RNAi
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture rock lobster
UTAS Author:Fitzgibbon, QP (Associate Professor Quinn Fitzgibbon)
UTAS Author:Smith, GG (Professor Gregory Smith)
ID Code:141077
Year Published:2020
Funding Support:Australian Research Council (IH190100014)
Web of Science® Times Cited:1
Deposited By:Fisheries and Aquaculture
Deposited On:2020-09-23
Last Modified:2021-02-15
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