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Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry


Chen, L and Cabot, JM and Sanz Rodriguez, E and Ghiasvand, A and Innis, PC and Paull, B, Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry, Analyst pp. 1-9. ISSN 0003-2654 (2020) [Refereed Article]

Copyright Statement

The Royal Society of Chemistry 2020

DOI: doi:10.1039/d0an01344g


The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct "on-thread" ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

Item Details

Item Type:Refereed Article
Keywords:thread-based isoelectric focusing, desorption electrospray ionization mass spectrometry
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Analytical spectrometry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Chen, L (Miss Liang Chen)
UTAS Author:Cabot, JM (Dr Joan Cabot Canyelles)
UTAS Author:Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez)
UTAS Author:Ghiasvand, A (Professor Alireza Ghiasvand)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:140965
Year Published:2020
Web of Science® Times Cited:9
Deposited By:Chemistry
Deposited On:2020-09-16
Last Modified:2022-08-22

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