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Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry
Citation
Chen, L and Cabot, JM and Sanz Rodriguez, E and Ghiasvand, A and Innis, PC and Paull, B, Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry, Analyst pp. 1-9. ISSN 0003-2654 (2020) [Refereed Article]
Copyright Statement
© The Royal Society of Chemistry 2020
Abstract
The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct "on-thread" ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.
Item Details
Item Type: | Refereed Article |
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Keywords: | thread-based isoelectric focusing, desorption electrospray ionization mass spectrometry |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Analytical spectrometry |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Chen, L (Miss Liang Chen) |
UTAS Author: | Cabot, JM (Dr Joan Cabot Canyelles) |
UTAS Author: | Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez) |
UTAS Author: | Ghiasvand, A (Professor Alireza Ghiasvand) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 140965 |
Year Published: | 2020 |
Web of Science® Times Cited: | 9 |
Deposited By: | Chemistry |
Deposited On: | 2020-09-16 |
Last Modified: | 2022-08-22 |
Downloads: | 0 |
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