The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct "on-thread" ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

Item Type:	Refereed Article
Keywords:	thread-based isoelectric focusing, desorption electrospray ionization mass spectrometry
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Chen, L (Miss Liang Chen)
UTAS Author:	Cabot, JM (Dr Joan Cabot Canyelles)
UTAS Author:	Sanz Rodriguez, E (Dr Estrella Sanz Rodriguez)
UTAS Author:	Ghiasvand, A (Professor Alireza Ghiasvand)
UTAS Author:	Paull, B (Professor Brett Paull)
ID Code:	140965
Year Published:	2020
Web of Science® Times Cited:	4
Deposited By:	Chemistry
Deposited On:	2020-09-16
Last Modified:	2020-10-16
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