eCite Digital Repository
Conversion of unstable compounds can contribute to the auxin pool during sample preparation
Citation
Gelinas-Marion, A and Nichols, DS and Ross, JJ, Conversion of unstable compounds can contribute to the auxin pool during sample preparation, Plant Physiology, 183, (4) pp. 1432-1434. ISSN 0032-0889 (2020) [Refereed Article]
Copyright Statement
Copyright 2020 American Society of Plant Biologists
Abstract
Auxin is a powerful regulator of plant growth and development (Heisler and Byrne, 2020). Accurate quantification of the main form of auxin, indole-3-acetic acid (IAA), is therefore essential for the study of auxin biology. Synthetic forms of the IAA-related compounds indole-3-pyruvic acid (IPyA) and indole-3-acetaldehyde (IAAld) have been shown to convert nonenzymatically to IAA, particularly in aqueous environments (Ernstsen et al., 1986; Tam and Normanly, 1998; Quittenden et al., 2009; Dai et al., 2013). These compounds are present in plants, and IPyA is now considered the main intermediate between tryptophan and IAA (Zhao, 2018). If substantial amounts of IPyA and/or IAAld convert to IAA during sample preparation, IAA quantification may be severely compromised. Here, a cysteamine-based derivatization method for converting IPyA and IAAld to stable forms (Novák et al., 2012) was used to demonstrate that endogenous IAA-related compounds can convert nonenzymatically to IAA during pea (Pisum sativum) and Arabidopsis (Arabidopsis thaliana) sample preparation. In derivatized samples, quantified IAA content was up to 40% lower than in underivatized controls. As a result, we recommend that harvested samples be derivatized soon after the completion of a short extraction period.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | plant hormone, auxin |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Environmentally sustainable plant production |
Objective Field: | Environmentally sustainable plant production not elsewhere classified |
UTAS Author: | Gelinas-Marion, A (Dr Ariane Gelinas-Marion) |
UTAS Author: | Nichols, DS (Dr David Nichols) |
UTAS Author: | Ross, JJ (Associate Professor John Ross) |
ID Code: | 140790 |
Year Published: | 2020 |
Web of Science® Times Cited: | 3 |
Deposited By: | Central Science Laboratory |
Deposited On: | 2020-09-08 |
Last Modified: | 2021-02-11 |
Downloads: | 0 |
Repository Staff Only: item control page