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Insulin-like signalling influences the coordination of larval hemocyte number with body size in Drosophila melanogaster
Citation
Bakopoulos, D and Forbes Beadle, L and Esposito, KM and Mirth, CK and Warr, CG and Johnson, TK, Insulin-like signalling influences the coordination of larval hemocyte number with body size in Drosophila melanogaster, G3: Genes, Genomes, Genetics, 10, (7) pp. 2213-2220. ISSN 2160-1836 (2020) [Refereed Article]
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Copyright Statement
Copyright © 2020 Bakopoulos et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1534/g3.120.401313
Abstract
Blood cells, known as hemocytes in invertebrates, play important and conserved roles in immunity, wound healing and tissue remodelling. The control of hemocyte number is therefore critical to ensure these functions are not compromised, and studies using Drosophila melanogaster are proving useful for understanding how this occurs. Recently, the embryonic patterning gene, torso-like (tsl), was identified as being required both for normal hemocyte development and for providing immunity against certain pathogens. Here, we report that Tsl is required specifically during the larval phase of hematopoiesis, and that tsl mutant larvae likely have reduced hemocyte numbers due to a reduced larval growth rate and compromised insulin signaling. Consistent with this, we find that impairing insulin-mediated growth, either by nutrient deprivation or genetically, results in fewer hemocytes. This is likely the result of impaired insulin-like signaling in the hemocytes themselves, since modulation of Insulin-like Receptor (InR) activity specifically in hemocytes causes concomitant changes to their population size in developing larvae. Taken together, our work reveals the strong relationship that exists between body size and hemocyte number, and suggests that insulin-like signaling contributes to, but is not solely responsible for, keeping these tightly aligned during larval development.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Drosophila melanogaster, hemocyte, insulin-like signalling, torso-like growth |
| Research Division: | Biological Sciences |
| Research Group: | Genetics |
| Research Field: | Developmental genetics (incl. sex determination) |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Warr, CG (Professor Coral Warr) |
| ID Code: | 140584 |
| Year Published: | 2020 |
| Web of Science® Times Cited: | 3 |
| Deposited By: | Office of the School of Medicine |
| Deposited On: | 2020-08-28 |
| Last Modified: | 2021-04-20 |
| Downloads: | 18 View Download Statistics |
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