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Advances in diagnosis of Tuberculosis: an update into molecular diagnosis of Mycobacterium tuberculosis
journal contribution
posted on 2023-05-20, 17:16 authored by Acharya, B, Acharya, A, Gautam, S, Ghimire, SP, Mishra, G, Parajuli, N, Sapkota, BTuberculosis (TB) is a major cause of deaths by a single infectious agent and has now been a global public health problem due to increasing numbers of drug-resistant cases. Early and effective treatment is crucial to prevent the emergence of drug-resistance strains. This demands the availability of fast and reliable point-of-care (POC) diagnostic methods for effective case management. Commonly used methods to screen and diagnose TB are clinical, immunological, microscopy, radiography, and bacterial culture. In addition, recent advances in molecular diagnostic methods including MTBDRplus, loop-mediated isothermal amplification (LAMP), line probe assay (LPA), GeneXpert, and whole genome sequencing (WGS) have been employed to diagnose and characterize TB. These methods can simultaneously identify Mycobacterium tuberculosis (MTB) and mutation(s) associated with routinely used anti-TB drugs. Here, we review the use of currently available diagnostic methods and strategies including conventional to recently implemented next-generation sequencing (NGS) methods used to detect MTB in clinical perspective.
History
Publication title
Molecular Biology ReportsVolume
47Issue
5Pagination
4065-4075ISSN
0301-4851Department/School
Tasmanian School of MedicinePublisher
Kluwer Academic PublPlace of publication
Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 GzRights statement
© Springer Nature B.V. 2020Repository Status
- Restricted