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Peanut applied to the skin of nonhuman primates induces antigen‐specific IgG but not IgE

Citation

Kulis, MD and Smeekens, JM and Kavanagh, K and Jorgensen, MJ, Peanut applied to the skin of nonhuman primates induces antigen‐specific IgG but not IgE, Immunity, Inflammation and Disease, 8 pp. 211-215. ISSN 2050-4527 (2020) [Refereed Article]


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Copyright 2020 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1002/iid3.296

Abstract

Introduction: Previous studies in humans support the dual‐allergen exposure hypothesis, and several studies in mouse models have demonstrated that cutaneous exposure to disrupted or intact skin can lead to sensitization to peanut. However, the field lacks definitive evidence that cutaneous exposure leads to peanut allergy in humans or other primates.

Methods: Peanut extract was applied to the shaved back of the neck of four male and four female African green monkeys three times per week for 4 weeks. An oral food challenge (OFC) was performed the following week by gavage of 200 mg of peanut protein, and vital signs were monitored for 30 minutes post‐OFC. Blood was collected at baseline, day 11, day 32, and 30 minutes post‐OFC. Total IgE, and peanut‐specific immunoglobulin E (IgE) and immunoglobulin G (IgG) were quantified in serum collected throughout the 4 weeks. Histamine was measured in serum collected 30 minutes post‐OFC.

Results: Peanut‐specific IgE was undetectable at any time points in any of the monkeys, and there was no consistent increase in total IgE. During the oral challenge, none of the monkeys experienced allergic symptoms and histamine levels did not change. However, seven of the eight monkeys produced increasing peanut‐specific IgG by day 32, indicating that repeated skin exposure to peanut is immunogenic.

Conclusions: Skin exposure to peanut did not lead to sensitization in this study, and monkeys did not experience anaphylaxis upon peanut challenge. However, monkeys produced increased peanut‐specific IgG throughout peanut exposure, indicating that repeated skin exposure to peanut is immunogenic.

Item Details

Item Type:Refereed Article
Keywords:African green monkey, cutaneous, food allergy, IgE, IgG, nonhuman primate, peanut allergy
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Allergy
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Kavanagh, K (Associate Professor Kylie Kavanagh)
ID Code:140330
Year Published:2020
Web of Science® Times Cited:3
Deposited By:Medicine
Deposited On:2020-08-07
Last Modified:2020-09-16
Downloads:2 View Download Statistics

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