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Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.)

Citation

Slinger, J and Adams, MB and Wynne, JW, Bacteriomic profiling of branchial lesions induced by Neoparamoeba perurans challenge reveals commensal dysbiosis and an association with Tenacibaculum dicentrarchi in AGD-affected Atlantic salmon (Salmo Salar L.), Microorganisms, 8, (8) pp. 1-17. ISSN 2076-2607 (2020) [Refereed Article]


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Copyright 2020 by the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/microorganisms8081189

Abstract

Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill associated commensal bacteria is unknown. A 16S rRNA sequencing approach was employed to profile changes in bacterial community composition, within amoebic gill disease (AGD)-affected and non-affected gill tissue. The bacterial diversity of biopsies with and without diseased tissue was significantly lower in the AGD-affected fish compared to uninfected fish. Furthermore, within the AGD-affected tissue, lesions appeared to contain a significantly higher abundance of the Flavobacterium, Tenacibaculum dicentrarchi compared to adjunct unaffected tissues. Quantitative PCR specific to both N. perurans and T. dicentrarchi was used to further examine the co-abundance of these known fish pathogens. A moderate positive correlation between these pathogens was observed. Taken together, the present study sheds new light on the complex interaction between the host, parasite and bacterial communities during AGD progression. The role that T. dicentrarchi may play in this complex relationship requires further investigation.

Item Details

Item Type:Refereed Article
Keywords:AGD, microbiomics, gills, amoeba, Neoparamoeba, AGD, bacteria, Atlantic salmon, bacteriome, gill microbiota, Tenacibaculum, Tenacibaculosis
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Slinger, J (Mr Joel Slinger)
UTAS Author:Adams, MB (Dr Mark Adams)
ID Code:140298
Year Published:2020
Web of Science® Times Cited:9
Deposited By:Fisheries and Aquaculture
Deposited On:2020-08-05
Last Modified:2021-02-10
Downloads:6 View Download Statistics

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