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Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

Citation

Shinde, T and Perera, AP and Vemuri, R and Gondalia, SV and Beale, DJ and Karpe, AV and Shastri, S and Basheer, W and Southam, B and Eri, R and Stanley, R, Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases, European Journal of Nutrition, 59, (8) pp. 3669-3689. ISSN 1436-6207 (2020) [Refereed Article]


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Copyright 2020 The Author(s)

DOI: doi:10.1007/s00394-020-02200-9

Abstract

Purpose: The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextran-sulfate sodium (DSS)-induced colitis in mice.

Methods: Eight-week-old C57BL/6 mice were fed standard rodent chow diet supplemented with either B. coagulans, GBRS or its synbiotic combination. After 7 days supplementation, colitis was induced by adding 2% DSS in drinking water for 7 days while continuing the supplemented diets. Animal health was monitored and after 14 days all animals were sacrifced to measure the biochemical and histochemical changes associated with each supplement type.

Results: The disease activity index and histological damage score for DSS-control mice (6.1, 17.1, respectively) were significantly higher (p < 0.0001) than the healthy mice. Synbiotic supplementation alleviated these markers (-67%, -94% respectively) more adequately than B. coagulans (-52%, -58% respectively) or GBRS (-57%, -26%, respectively) alone. Compared to DSS-control synbiotic supplementation significantly (p<0.0001) maintained expressions of tight junction proteins. Moreover, synbiotic effects accounted for ∼40% suppression of IL-1β and ∼29% increase in IL-10 levels in serum while also reducing C-reactive protein (-37%) compared to that of the DSS-control. While, B. coagulans alone could not induce additional levels of short-chain fatty acid (SCFA) production beyond the caecum, the synbiotic combination with GBRS resulted in substantial increased SCFA levels across the whole length of the colon.

Conclusion: The synbiotic supplementation with B. coagulans and GBRS ameliorated the overall inflammatory status of the experimental IBD model via synergistic functioning. This supports researching its application in mitigating inflammation in human IBD.

Item Details

Item Type:Refereed Article
Keywords:synbiotic, prebiotic, probiotic, infammatory bowel diseases, bacillus spores, green banana, resistant starch, mucosal barrier, short-chain fatty acids
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Gastroenterology and hepatology
Objective Division:Manufacturing
Objective Group:Processed non-food agriculture products (excl. wood, paper and fibre)
Objective Field:Plant extracts
UTAS Author:Shinde, T (Ms Tanvi Shinde)
UTAS Author:Perera, AP (Mrs Agampodi Perera)
UTAS Author:Vemuri, R (Mr Ravichandra Vemuri)
UTAS Author:Shastri, S (Mrs Sonia Shastri)
UTAS Author:Basheer, W (Dr Waheedha Basheer)
UTAS Author:Southam, B (Mr Benjamin Southam)
UTAS Author:Eri, R (Associate Professor Raj Eri)
UTAS Author:Stanley, R (Professor Roger Stanley)
ID Code:140078
Year Published:2020
Web of Science® Times Cited:12
Deposited By:Health Sciences
Deposited On:2020-07-23
Last Modified:2021-02-12
Downloads:2 View Download Statistics

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