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Development of EST microsatellite markers for the Tasmanian palaeoendemic conifer Lagarostrobos franklinii (Hook. f.) Quinn (Podocarpaceae)
Citation
Marthick, JR and Larcombe, MJ and Worth, JRP, Development of EST microsatellite markers for the Tasmanian palaeoendemic conifer Lagarostrobos franklinii (Hook. f.) Quinn (Podocarpaceae), Silvae Genetica, 69, (1) pp. 1-5. ISSN 0037-5349 (2020) [Refereed Article]
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Copyright Statement
Copyright 2020 The Authors. Licensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) https://creativecommons.org/licenses/by-nc-nd/3.0/
Abstract
Nuclear Expressed Sequence Tag (EST) microsatellite markers were developed for the Tasmanian palaeoendemic conifer Lagarostrobos franklinii (Hook.-f.) Quinn for genetic studies. RNAseq data was mined for EST microsatellites, and primer pairs were synthesised from 70 contigs with 50 producing amplification products. Of these 50, 10 reliably amplified and displayed polymorphism across 8 samples representing the entire species range. The genetic diversity of these 10 loci was then examined in three wild populations (84 samples). The number of alleles varied from two to thirteen per locus with the average number of alleles per population ranging between 3.0-4.7. Observed and expected heterozygosity ranged from 0.34-0.42 and 0.37-0.44, respectively. Marker cross-amplification was tested in the New Zealand sister species Manoao colensoi (Hook. f.) Molloy, but no markers amplified reliably, which possibly reflects the age of divergence between these species (~64 million years). These are the first microsatellite markers developed for the monotypic genus Lagarostrobos. They will be valuable for assessing the species extant genetic diversity, the impact of past climatic perturbations and human disturbance and the role of clonal propagation in recruitment.
Item Details
Item Type: | Refereed Article |
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Keywords: | Lagarostrobos, Podocarpaceae, EST microsatellites, palaeoendemic, next generation sequencing, huon pine, Tasmania |
Research Division: | Biological Sciences |
Research Group: | Genetics |
Research Field: | Genetics not elsewhere classified |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Marthick, JR (Mr James Marthick) |
ID Code: | 139916 |
Year Published: | 2020 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2020-07-15 |
Last Modified: | 2020-08-21 |
Downloads: | 11 View Download Statistics |
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