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Nuclear Expressed Sequence Tag (EST) microsatellite markers were developed for the Tasmanian palaeoendemic conifer Lagarostrobos franklinii (Hook.-f.) Quinn for genetic studies. RNAseq data was mined for EST microsatellites, and primer pairs were synthesised from 70 contigs with 50 producing amplification products. Of these 50, 10 reliably amplified and displayed polymorphism across 8 samples representing the entire species range. The genetic diversity of these 10 loci was then examined in three wild populations (84 samples). The number of alleles varied from two to thirteen per locus with the average number of alleles per population ranging between 3.0-4.7. Observed and expected heterozygosity ranged from 0.34-0.42 and 0.37-0.44, respectively. Marker cross-amplification was tested in the New Zealand sister species Manoao colensoi (Hook. f.) Molloy, but no markers amplified reliably, which possibly reflects the age of divergence between these species (~64 million years). These are the first microsatellite markers developed for the monotypic genus Lagarostrobos. They will be valuable for assessing the species extant genetic diversity, the impact of past climatic perturbations and human disturbance and the role of clonal propagation in recruitment.

Item Type:	Refereed Article
Keywords:	Lagarostrobos, Podocarpaceae, EST microsatellites, palaeoendemic, next generation sequencing, huon pine, Tasmania
Research Division:	Biological Sciences
Research Group:	Genetics
Research Field:	Genetics not elsewhere classified
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the biological sciences
UTAS Author:	Marthick, JR (Mr James Marthick)
ID Code:	139916
Year Published:	2020
Deposited By:	Menzies Institute for Medical Research
Deposited On:	2020-07-15
Last Modified:	2020-08-21
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