University of Tasmania
Browse

File(s) under permanent embargo

Rapid method to obtain high quality nuclear and chromosome images directly from living cells in the Characeae

journal contribution
posted on 2023-05-20, 15:56 authored by Gargiulo, GM, Gemelli, F, Cambrea, G, Ferraro, GB, Crosca, A
This study focused on a method based on the capacity of cationic dyes to stain only the nucleus and chromosomes in cells subjected to either acid or alkaline hydrolysis. The method and the squash were optimized for the Characeae and were described in detail. Nuclei from vegetative shoot apices and antheridial filament cells of unfixed, fixed and herbarium material of Nitella opaca were investigated using the Azure A or Toulidine Blue stains. Comparisons with some other staining methods, used in the previous studies, were also reported. The Azure A/Toulidine Blue method is useful to obtain clear images of chromosome morphology comparable or higher to that obtained with Feulgen or Aceto-Orcein. It requires little time and a less complicated procedure in comparison with other staining methods.

History

Publication title

Phycological Research

Volume

67

Pagination

72-76

ISSN

1440-1835

Department/School

Institute for Marine and Antarctic Studies

Publisher

Wiley-Blackwell Publishing Asia

Place of publication

Australia

Rights statement

Copyright 2018 Japanese Society of Phycology

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC