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A specific mutation in Muc2 determines early dysbiosis in colitis-prone Winnie mice

Citation

Liso, M and De Santis, S and Verna, G and Dicarlo, M and Calasso, M and Santino, A and Gigante, I and Eri, R and Raveenthiraraj, S and Sobolewski, A and Palmitessa, V and Lippolis, A and Mastronardi, M and Armentano, R and Serino, G and De Angelis, M and Chieppa, M, A specific mutation in Muc2 determines early dysbiosis in colitis-prone Winnie mice, Inflammatory Bowel Diseases, 26, (4) pp. 546-556. ISSN 1078-0998 (2020) [Refereed Article]


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Copyright Statement

© 2019 Crohn’s & Colitis Foundation. Licensed under Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/

DOI: doi:10.1093/ibd/izz279

Abstract

Background: Inflammatory bowel disease (IBD), including Crohn disease (CD) and ulcerative colitis (UC), is a multifactorial disorder characterized by chronic inflammation and altered gut barrier function. Dysbiosis, a condition defined by dysregulation of the gut microbiome, has been reported in patients with IBD and in experimental models of colitis. Although several factors have been implicated in directly affecting gut microbial composition, the genetic determinants impacting intestinal dysbiosis in IBD remain relatively unknown.

Methods: We compared the microbiome of normal, uninflamed wild-type (WT) mice with that of a murine model of UC (ie, Winnie strain). Winnie mice possess a missense mutation in Muc2 that manifests in altered mucus production as early as 4 weeks of age, with ensuing colonic inflammation. To better address the potential role of mutant Muc2 in promoting dysbiosis in Winnie mice, we evaluated homozygous mutant mice (Winnie-/-) with their WT littermates that, after weaning from common mothers, were caged separately according to genotype. Histologic and inflammatory status were assessed over time, along with changes in their respective microbiome compositions.

Results: Dysbiosis in Winnie mice was already established at 4 weeks of age, before histologic evidence of gut inflammatory changes, in which microbial communities diverged from that derived from their mothers. Furthermore, dysbiosis persisted until 12 weeks of age, with peak differences in microbiome composition observed between Winnie and WT mice at 8 weeks of age. The relative abundance of Bacteroidetes was greater in Winnie compared with WT mice. Verrucomicrobia was detected at the highest relative levels in 4-week-old Winnie mice; in particular, Akkermansia muciniphila was among the most abundant species found at 4 weeks of age.

Conclusions: Our results demonstrate that mutant genetic determinants involved in the complex regulation of intestinal homeostasis, such as that observed in Winnie mice, are able to promote early gut dysbiosis that is independent from maternal microbial transfer, including breastfeeding. Our data provide evidence for intestinal dysbiosis attributed to a Muc2-driven mucus defect that leads to colonic inflammation and may represent an important target for the design of future interventional studies.

Item Details

Item Type:Refereed Article
Keywords:microbiome, inflammatory bowel disease (IBD), dysbiosis, murine model of ulcerative colitis (UC)
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Gastroenterology and hepatology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Eri, R (Associate Professor Raj Eri)
ID Code:139805
Year Published:2020
Web of Science® Times Cited:13
Deposited By:Health Sciences
Deposited On:2020-07-06
Last Modified:2020-08-20
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