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Cow dung biomass smoke exposure increases adherence of respiratory pathogen nontypeable Haemophilus infuenzae to human bronchial epithelial cells
journal contribution
posted on 2023-05-20, 15:52 authored by Rajendra KCRajendra KC, Hyland, IK, Jason SmithJason Smith, Shukla, SD, Hansbro, PM, Graeme ZoskyGraeme Zosky, Gunasegaran KarupiahGunasegaran Karupiah, O'Toole, RFBiomass smoke exposure is associated with a heightened risk of development of respiratory diseases that include chronic obstructive pulmonary disease (COPD). The aim of this study was to increase our understanding of how biomass smoke could contribute to an increased susceptibility to respiratory infection. We investigated the efects of cow dung and wood smoke exposure on human bronchial epithelial cells with respect to adherence of a major respiratory bacterial pathogen in COPD, nontypeable Haemophilus infuenzae (NTHi), using immunofuorescence microscopy. In addition, expression of a known receptor of NTHi, platelet-activating factor receptor (PAFR), and two pro-infammatory cytokines, interleukin 6 (IL6) and interleukin-8 (IL-8), were determined using quantitative polymerase chain reaction. We observed a dose-dependent increase in NTHi adhesion to human bronchial epithelial cells following exposure to cow dung but not wood smoke extracts. Pre-treatment with PAFR antagonists, WEB-2086 and its analogue, C17, decreased adherence by NTHi to airway epithelial cells exposed to cow dung smoke. Both cow dung and wood smoke-induced expression of PAFR, as well as of IL-6 and IL-8, which was inhibited by WEB-2086 and C17. In conclusion, biomass smoke from combustion of cow dung and woodinduced expression of PAFR and airway infammatory markers in human bronchial epithelial cells. Cow dung exposure, but not wood smoke exposure, mediated a measurable increase in NTHi adhesion to airway epithelial cells that was inhibited by PAFR antagonists. This work highlights the potential of PAFR as a therapeutic target for reducing the impact of hazardous biomass smoke exposure on respiratory health.
History
Publication title
Exposure and HealthVolume
12Pagination
883-895ISSN
2451-9766Department/School
Tasmanian School of MedicinePublisher
Springer NetherlandsPlace of publication
NetherlandsRights statement
© Springer Nature B.V. 2020Repository Status
- Restricted