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Quantification of muco-obstructive lung disease variability in mice via laboratory X-ray velocimetry

Citation

Werdiger, F and Donnelley, M and Dubsky, S and Murrie, RP and Carnibella, RP and Samarage, CR and How, YY and Zosky, GR and Fouras, A and Parsons, DW and Morgan, KS, Quantification of muco-obstructive lung disease variability in mice via laboratory X-ray velocimetry, Scientific Reports, 10, (1) Article 10859. ISSN 2045-2322 (2020) [Refereed Article]


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Copyright 2020 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1038/s41598-020-67633-y

Abstract

To effectively diagnose, monitor and treat respiratory disease clinicians should be able to accurately assess the spatial distribution of airflow across the fine structure of lung. This capability would enable any decline or improvement in health to be located and measured, allowing improved treatment options to be designed. Current lung function assessment methods have many limitations, including the inability to accurately localise the origin of global changes within the lung. However, X-ray velocimetry (XV) has recently been demonstrated to be a sophisticated and non-invasive lung function measurement tool that is able to display the full dynamics of airflow throughout the lung over the natural breathing cycle. In this study we present two developments in XV analysis. Firstly, we show the ability of laboratory-based XV to detect the patchy nature of cystic fibrosis (CF)-like disease in β-ENaC mice. Secondly, we present a technique for numerical quantification of CF-like disease in mice that can delineate between two major modes of disease symptoms. We propose this analytical model as a simple, easy-to-interpret approach, and one capable of being readily applied to large quantities of data generated in XV imaging. Together these advances show the power of XV for assessing local airflow changes. We propose that XV should be considered as a novel lung function measurement tool for lung therapeutics development in small animal models, for CF and for other muco-obstructive diseases.

Item Details

Item Type:Refereed Article
Keywords:mucous, x-ray velocimetry, synchrotron
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:139795
Year Published:2020
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-07-06
Last Modified:2020-08-04
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