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Children with nut allergies have impaired gene expression of Toll-like receptors pathway

Citation

Poole, A and Song, Y and O'Sullivan, M and Lee, KH and Metcalfe, J and Guo, J and Brown, H and Mullins, B and Loh, R and Zhang, GB, Children with nut allergies have impaired gene expression of Toll-like receptors pathway, Pediatric Allergy and Immunology pp. 1-7. ISSN 0905-6157 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 EAACI and John Wiley and Sons A/S

DOI: doi:10.1111/pai.13246

Abstract

Background: Trends in food allergies prompted investigation into the underlying mechanisms. Genetic and epigenetic factors are of high interest, and, in particular, the interplay between genes relating to immune factors directly and indirectly involved in food allergy pathogenesis. We sought to determine potential links between gene expression and epigenetic factors relating to Toll-like receptor (TLR) pathways and childhood food allergies.

Methods: In a cross-sectional study, samples from 80 children with and without food allergies were analysed for gene expression, DNA methylation and a range of immune factors relating to TLR pathways. TLR2, TLR4, CD14, IL5, IL13 and vitamin D were explored.

Results: The importance of these immune factors appeared to vary between the different types of food allergies. Expression of TLR2 (P < .001), TLR4 (P = .014) and CD14 (P = .028) varied significantly between children with no food allergy, allergy to nuts and peanuts, and allergy to eggs. DNA methylation in the promoter regions of these genes had a significant association with gene expression. These trends persisted when subjects were stratified by nut allergy vs no nut allergy. Furthermore, TLR2 (P = .001) and CD14 (P = .007) expressions were significantly lower in children with food allergies when compared to those without.

Conclusion: Gene expression of TLR pathway genes was directly related to food allergy type, and DNA methylation had an indirect effect. TLR2 pathways are of significant interest in nut allergies.

Item Details

Item Type:Refereed Article
Keywords:immune dysfunction, DNA methylation, Toll-like receptor, epigenetic factors, food allergy, gene expression, nut allergy
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Song, Y (Dr Yong Song)
ID Code:139652
Year Published:2020
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-06-24
Last Modified:2020-08-28
Downloads:0

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