Poole, A and Song, Y and O'Sullivan, M and Lee, KH and Metcalfe, J and Guo, J and Brown, H and Mullins, B and Loh, R and Zhang, GB, Children with nut allergies have impaired gene expression of Toll-like receptors pathway, Pediatric Allergy and Immunology pp. 1-7. ISSN 0905-6157 (2020) [Refereed Article]
Copyright 2020 EAACI and John Wiley and Sons A/S
Methods: In a cross-sectional study, samples from 80 children with and without food allergies were analysed for gene expression, DNA methylation and a range of immune factors relating to TLR pathways. TLR2, TLR4, CD14, IL5, IL13 and vitamin D were explored.
Results: The importance of these immune factors appeared to vary between the different types of food allergies. Expression of TLR2 (P < .001), TLR4 (P = .014) and CD14 (P = .028) varied significantly between children with no food allergy, allergy to nuts and peanuts, and allergy to eggs. DNA methylation in the promoter regions of these genes had a significant association with gene expression. These trends persisted when subjects were stratified by nut allergy vs no nut allergy. Furthermore, TLR2 (P = .001) and CD14 (P = .007) expressions were significantly lower in children with food allergies when compared to those without.
Conclusion: Gene expression of TLR pathway genes was directly related to food allergy type, and DNA methylation had an indirect effect. TLR2 pathways are of significant interest in nut allergies.
|Item Type:||Refereed Article|
|Keywords:||immune dysfunction, DNA methylation, Toll-like receptor, epigenetic factors, food allergy, gene expression, nut allergy|
|Research Division:||Biomedical and Clinical Sciences|
|Research Group:||Cardiovascular medicine and haematology|
|Research Field:||Respiratory diseases|
|Objective Group:||Clinical health|
|Objective Field:||Clinical health not elsewhere classified|
|UTAS Author:||Song, Y (Dr Yong Song)|
|Web of Science® Times Cited:||7|
|Deposited By:||Menzies Institute for Medical Research|
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