eCite Digital Repository

Cloned ermTR gene confers low level erythromycin but high level clindamycin resistance in Streptococcus pyogenes NZ131

Citation

Oryasin, E and Biyik, HH and Tristram, S and Bozdogan, B, Cloned ermTR gene confers low level erythromycin but high level clindamycin resistance in Streptococcus pyogenes NZ131, Microbial Drug Resistance, 26, (7) pp. 747-751. ISSN 1076-6294 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 Mary Ann Liebert, Inc.

DOI: doi:10.1089/mdr.2019.0133

Abstract

Objectives: The most common macrolide resistance mechanisms in streptococci are the presence of methylase encoding genes ermB and ermTR or the presence of efflux encoded by mef genes. In the present study we aimed to show the effects of the ermTR gene under isogenic conditions on the activities of macrolides and lincosamides in streptococci.

Materials and Methods: Total DNA was extracted from Streptococcus pyogenes C1, and the ermTR gene was amplified with or without the regulatory region using modified primer with insertion of restriction sites to clone in to pUC18. Transformants were selected after electroporation of Escherichia coli DB10. The recombinant plasmids were purified and merged to pJIM2246 to transform Gram positive bacteria. Recombinant pJIM2246 plasmids with the ermTR gene were then introduced into S. pyogenes NZ131 by electroporation.

Results: After transformation with ermTR without regulatory region the minimal inhibitory concentration (MIC) for erythromycin and clindamycin increased from ≤0.06 to ≤0.06 to 8 and >128 mg/L, respectively. Induction with erythromycin affected the MICs for clindamycin of S. pyogenes transformed with ermTR with the regulatory region. Double disk testing showed that induction with erythromycin and azithromycin for the S. pyogenes transformed with ermTR, and regulatory regions decreased the clindamycin inhibition zone but not telithromycin. The ermTR gene in isogenic conditions confers low level resistance to erythromycin and high level resistance to clindamycin.

Conclusion: The different induction and resistance profiles of ermTR compared to other erm genes suggest that the methylation of ErmTR may be different than well studied methylases.

Item Details

Item Type:Refereed Article
Keywords:cloning, ermTR, macrolide resistance
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Tristram, S (Dr Stephen Tristram)
ID Code:139298
Year Published:2020
Deposited By:Health Sciences
Deposited On:2020-06-09
Last Modified:2021-05-25
Downloads:0

Repository Staff Only: item control page