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Epigenetic regulation of the ITGB4 gene in prostate cancer

Citation

Wilkinson, EJ and Woodworth, AM and Parker, M and Phillips, JL and Malley, RC and Dickinson, JL and Holloway, AF, Epigenetic regulation of the ITGB4 gene in prostate cancer, Experimental Cell Research pp. 1-10. ISSN 0014-4827 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 Elsevier Inc.

DOI: doi:10.1016/j.yexcr.2020.112055

Abstract

Background: Examination of epigenetic changes at the ITGB4 gene promoter reveals altered methylation at different stages of prostate tumour progression and these changes may, in part, explain the complex patterns of gene expression of this integrin observed. Transcriptional re-programming perturbs expression of cell adhesion molecules and underpins metastatic tumour cell behaviour. Decreasing expression of the cell adhesion molecule ITGB4, which encodes the beta subunit of the integrin, alpha6 beta4 (α6β4), has been correlated with increased tumour aggressiveness and metastasis in multiple tumour types including prostate cancer. Paradoxically, in vitro studies in tumour cell models demonstrate that ITGB4 mediates cell mobility and invasion. Herein we examined whether transcriptional re-programming by methylation influenced ITGB4 gene expression at different stages of prostate cancer progression. Bisulphite sequencing of a large CpG island in the ITGB4 gene promoter identified differentially methylated regions in prostate cancer cell lines representing a localised tumour (22Rv1), lymph node metastasis (LNCaP), and a bone metastasis (PC-3). The highest levels of methylation were observed in the CpG island surrounding the ITGB4 transcription start site in PC-3 cells, and this observation also correlated with higher gene expression of ITGB4 in these cells. Furthermore, PC-3 cells expressed two distinct transcripts, using an alternate transcription start site, which was not detected in other cell lines. In prostate tumour biopsy samples, patterns of methylation across the ITGB4 promoter were similar overall in matched primary and metastatic samples (n = 4 pairs), with a trend toward loss of methylation at specific sites in metastatic lesions.

Item Details

Item Type:Refereed Article
Keywords:epigenetics, prostate cancer, integrins, metastasis, prostate cancer, integrins, DNA methylation, epigenetic regulation, metastasis
Research Division:Medical and Health Sciences
Research Group:Oncology and Carcinogenesis
Research Field:Cancer Genetics
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
UTAS Author:Wilkinson, EJ (Ms Emma Wilkinson)
UTAS Author:Woodworth, AM (Ms Alexandra Woodworth)
UTAS Author:Parker, M (Ms Madeline Parker)
UTAS Author:Phillips, JL (Dr Jessica Phillips)
UTAS Author:Malley, RC (Dr Roslyn Malley)
UTAS Author:Dickinson, JL (Professor Joanne Dickinson)
UTAS Author:Holloway, AF (Associate Professor Adele Holloway)
ID Code:138898
Year Published:2020
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-05-09
Last Modified:2020-06-18
Downloads:0

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