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Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation
Dorantes-Aranda, JJ and Hayashi, A and Turnbull, AR and Jolley, JYC and Harwood, DT and Hallegraeff, GM, Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation, Journal of AOAC International, 103, (3) pp. 784-791. ISSN 1060-3271 (2020) [Refereed Article]
Copyright 2020 AOAC International
Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money.
The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster.
The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4).
The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences.
The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster.
The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.
|Item Type:||Refereed Article|
|Keywords:||PST test kit validation, rock lobster|
|Research Division:||Agricultural, Veterinary and Food Sciences|
|Research Group:||Fisheries sciences|
|Research Field:||Fisheries management|
|Objective Division:||Animal Production and Animal Primary Products|
|Objective Group:||Fisheries - wild caught|
|Objective Field:||Wild caught rock lobster|
|UTAS Author:||Dorantes-Aranda, JJ (Dr Juan Dorantes Aranda)|
|UTAS Author:||Hayashi, A (Ms Aiko Hayashi)|
|UTAS Author:||Turnbull, AR (Dr Alison Turnbull)|
|UTAS Author:||Hallegraeff, GM (Professor Gustaaf Hallegraeff)|
|Web of Science® Times Cited:||1|
|Deposited By:||Ecology and Biodiversity|
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